MULTI SCIENCES
Mouse Osteopontin/OPN Cytokine ELISA Kit
Mouse Osteopontin/OPN Cytokine ELISA Kit
SKU:EK2135
Product Details
Brand | MultiSciences |
---|---|
CatNum | 70-EK2135 |
Product Name | Mouse osteopontin/OPN ELISA Kit |
Customs Name | Mouse osteopontin/OPN ELISA Kit |
Application | ELISA |
Reactivity | Mouse |
Assay Type | Sandwich ELISA |
Suitable Sample Type | serum, plasma, cell culture supernates |
Format | 96-well strip plate |
Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
Shipping Condition | 4℃ |
Sample Volume | 10 μl (prediluted) |
Sensitivity | 0.05 pg/ml |
Standard Curve Range | 15.63 - 1000 pg/ml |
CV of Intra plate | 1.8 % - 3.4 % |
CV of Inter plate | 2.1 % - 4.7 % |
Components | 96-well polystyrene microplate coated with a monoclonal antibody against OPN Mouse OPN Standard, lyophilized OPN Detect Antibody Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse OPN. The Mouse osteopontin/OPN ELISA is for research use only. Not for diagnostic or therapeutic procedures. Osteopontin (OPN) (also known as BSP-1, ETA-1, SPP-1 or Ric) encoded by the SPP1 gene is expressed mainly in bone. Regulation of the OPN gene is incompletely understood. Different cell types may differ in their regulatory mechanisms of the OPN gene. OPN has biological function in biomineralization, bone remodeling, immune functions, chemotaxis, cell activation and apoptosis, OPN also is associated with increased myocyte apoptosis and myocardial dysfunction. OPN is associated with many physiological and pathological processes including wound healing, bone turnover, tumorigenesis, inflammation, ischemia, and immune responses. Manipulation of plasma (or local) OPN levels may be useful in the treatment of autoimmune diseases, cancer metastasis, bone (and tooth) mineralization diseases, osteoporosis, and some forms of stress. |
