MULTI SCIENCES
Mouse RAGE ELISA Kit
Mouse RAGE ELISA Kit
SKU:EK2103
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK2103-96 |
| CatNum | EK2103-96 |
| Product Name | Mouse RAGE ELISA Kit |
| Customs Name | Mouse RAGE ELISA Kit |
| Product Spec | 96T |
| SalePrice | 3200 |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Mouse |
| Modality | NoData |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sensitivity | 7.90 pg/ml |
| Standard Curve Range | 15.63-1000 pg/ml |
| Spike Recovery Range | 97%-125% |
| Mean Spike Recovery | 105% |
| CV of Intra plate | 2.0 % - 3.4 % |
| CV of Inter plate | 2.2% - 4.8% |
| Components |
96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against mouse RAGE Mouse RAGE Standard, lyophilized RAGE Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate Stop Solution Washing Buffer (20×) Adhesive Films |
| PRINCIPLE OF THE ASSAY | Mouse RAGE ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse RAGE in the samples. An antibody specific for mouse RAGE has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for RAGE, and RAGE present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of RAGE bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader. |
| Describtion | Receptor for advanced glycation end product (RAGE) is a 35 kDa transmembrane receptor of the immunoglobulin super family. Its name comes from its ability to bind advanced glycation endproducts (AGE). In view of its inflammatory function in innate immunity and its ability to detect a class of ligands through a common motif, RAGE is often referred to as a pattern recognition receptor. RAGE has been linked to several chronic diseases, such as atherosclerosis, peripheral vascular disease, myocardial infarction, congestive heart failure, diabetes, Alzheimer's disease and so on. RAGE is expressed at the highest levels in the lung compared to other tissues, in particular in alveolar epithelial type I cells, and is lost in Idiopathic pulmonary fibrosis (IPF) indicating that expression and regulation of RAGE in the pulmonary system differs from that in the vascular system. Blockade/knockdown of RAGE resulted in impaired cell adhesion, and increased cell proliferation and migration. |
