MULTI SCIENCES
Mouse SAA ELISA Kit
Mouse SAA ELISA Kit
SKU:EK2279
Product Details
| Brand | MultiSciences |
|---|---|
| Product Code | 70-EK2279-96 |
| CatNum | EK2279-96 |
| Product Name | Mouse SAA ELISA Kit |
| Customs Name | Mouse SAA ELISA Kit |
| Product Spec | 96 T |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Mouse |
| SDS | SDS - EK2279 |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 100 μl |
| Sensitivity | 5.12pg/ml |
| Standard Curve Range | 156.25 - 10000 pg/ml |
| Spike Recovery Range | 90 % - 102 % |
| Mean Spike Recovery | 94% |
| CV of Intra plate | 1.9 % - 2.1 % |
| CV of Inter plate | 2.0 % - 2.4 % |
| Components |
96-well polystyrene microplate coated with a monoclonal antibody against SAA Human SAA Standard, lyophilized SAA Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for mouse SAA has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for SAA are pipetted into the wells and SAA present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, Streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of SAA bound in the initial step. The color development is stopped and the intensity of the color is measured. |
