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Factory Name MultiSciences
Product Code 70-EK2210-96
CatNum EK2210-96
Product Name Mouse SCF ELISA Kit
Customs Name Mouse SCF ELISA Kit
Product Spec 96T
SalePrice 3200
Type ELISA Kit
Application ELISA
Reactivity Mouse
Modality NoData
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sensitivity 0.17 pg/ml
Standard Curve Range 7.81 - 500 pg/ml
Spike Recovery Range 80 %-100 %
Mean Spike Recovery 89%
CV of Intra plate 2.6 % - 4.1 %
CV of Inter plate 3.4 % - 4.6 %

96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against mouse SCF

Mouse SCF Standard, lyophilized

SCF Detect Antibody

Standard Diluent


Assay Buffer (10×)


Stop Solution

Washing Buffer (20×)

Adhesive Films

PRINCIPLE OF THE ASSAY Mouse SCF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse SCF in the samples. An antibody specific for mouse SCF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and SCF present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for SCF is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of SCF bound in the initial step. The color development is stopped and the intensity of the color is measured.

Stem Cell Factor (SCF) is a cytokine that binds to the c-Kit receptor. SCF can exist both as a transmembrane protein and a soluble protein. SCF promotes mast cell adhesion, migration, proliferation, and survival. It also promotes the release of histamine and tryptase, which are involved in the allergic response. SCF acts in a synergistic manner with various growth factors, such as IL-3, IL-6, IL-11, GM-CSF, G-CSF, and Epo to induce myeloid and erythroid lineage colony formation. This cytokine plays an important role in hematopoiesis (formation of blood cells), spermatogenesis, and melanogenesis.

Cardiomyocyte-specific overexpression of transmembrane SCF promotes stem cell migration and improves cardiac function and animal survival after myocardial infarction. Mutations in this gene in dogs suggest it is a candidate skin cancer susceptibility gene.