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Mouse SP-Selectin/CD62P ELISA Kit

Mouse SP-Selectin/CD62P ELISA Kit


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Mouse SP-Selectin/CD62P ELISA Kit

Factory Name MultiSciences
Product Code 70-EK286-96
CatNum EK286-96
Product Name Mouse sP-Selectin/CD62P ELISA Kit
Customs Name Mouse sP-Selectin/CD62P ELISA Kit
Product Spec 96T
SalePrice 3200
Type ELISA Kit
Application ELISA
Reactivity Mouse
Modality NoData
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sensitivity 0.08 pg/ml
Standard Curve Range 23.44-1500 pg/ml
Spike Recovery Range 85%-113%
Mean Spike Recovery 113%
CV of Intra plate 1.6 % - 3.4 %
CV of Inter plate 1.9 % - 4.9 %

96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against mouse sP-Selectin

Mouse sP-Selectin Standard, lyophilized

sP-Selectin Detect Antibody


Assay Buffer (10×)


Stop Solution

Washing Buffer (20×)

Adhesive Films


Mouse sP-Selectin/CD62P ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse sP-Selectin in the samples. An antibody specific for mouse sP-Selectin has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for sP-Selectin, and sP-Selectin present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of sP-Selectin bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.


P-Selectin, also known as CD62P, Granule Membrane Protein 140 (GMP-140), and Platelet Activation-Dependent Granule to External Membrane Protein (PADGEM), is a cell surface glycoprotein that plays a critical role in the migration of lymphocytes into tissues. P-Selectin consists of an EGF-like domain, an NH2-terminal lectin type C domain, a transmembrane domain, a short cytoplasmic domain, and nine complement control domains. Evidence indicates that P-Selectin is mobilized to the cell surface in response to a variety of inflammatory or thrombogenic agents. Circulating soluble P-Selectin (sP-Selectin) is slightly smaller than native P-Selectin. Evidence suggests that the majority of sP-Selectin lacks the transmembrane anchoring domain due to its alternatively spliced mRNA. Monitoring of sP-Selectin levels in serum provides more detailed insights in several pathological situations such as thrombotic thrombocytopenic purpura and haemolytic uremic syndrome.