96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against mouse sP-Selectin

Mouse sP-Selectin Standard, lyophilized

sP-Selectin Detect Antibody

Streptavidin-HRP                                                                                           

Assay Buffer (10×)

Substrate

Stop Solution

Washing Buffer (20×)

Adhesive Films

Mouse sP-Selectin/CD62P ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse sP-Selectin in the samples. An antibody specific for mouse sP-Selectin has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for sP-Selectin, and sP-Selectin present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of sP-Selectin bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

P-Selectin, also known as CD62P, Granule Membrane Protein 140 (GMP-140), and Platelet Activation-Dependent Granule to External Membrane Protein (PADGEM), is a cell surface glycoprotein that plays a critical role in the migration of lymphocytes into tissues. P-Selectin consists of an EGF-like domain, an NH2-terminal lectin type C domain, a transmembrane domain, a short cytoplasmic domain, and nine complement control domains. Evidence indicates that P-Selectin is mobilized to the cell surface in response to a variety of inflammatory or thrombogenic agents. Circulating soluble P-Selectin (sP-Selectin) is slightly smaller than native P-Selectin. Evidence suggests that the majority of sP-Selectin lacks the transmembrane anchoring domain due to its alternatively spliced mRNA. Monitoring of sP-Selectin levels in serum provides more detailed insights in several pathological situations such as thrombotic thrombocytopenic purpura and haemolytic uremic syndrome.