MULTI SCIENCES
Mouse SVCAM-1/CD106 ELISA Kit
Mouse SVCAM-1/CD106 ELISA Kit
SKU:EK290
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK290-96 |
| CatNum | EK290-96 |
| Product Name | Mouse sVCAM-1/CD106 ELISA Kit |
| Customs Name | Mouse sVCAM-1/CD106 ELISA Kit |
| Product Spec | 96T |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Mouse |
| Modality | NoData |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 50ul |
| Sensitivity | 0.03 pg/ml |
| Standard Curve Range | 7.81-500 pg/ml |
| Spike Recovery Range | 85%-118% |
| Mean Spike Recovery | 98% |
| CV of Intra plate | 2.4% - 4.8 % |
| CV of Inter plate | 1.9% - 3.8 % |
| Components |
96-well polystyrene microplate coated with a monoclonal antibody against Mouse sVCAM-1 Mouse sVCAM-1 Standard, lyophilized Mouse sVCAM-1 Detect Antibody Streptavidin-HRP Amplification Reagent Concentrate Amplification Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| PRINCIPLE OF THE ASSAY | Mouse sVCAM-1/CD106 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse sVCAM-1 in the samples. An antibody specific for mouse sVCAM-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for sVCAM-1, and sVCAM-1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of sVCAM-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader. |
| Describtion |
Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a 100 - 110 kDa, type I transmembrane glycoprotein typically characterized by the presence of seven C2-type immunoglobulin (Ig) domains and functions as a cell adhesion molecule. The VCAM-1 protein mediates the adhesion of lymphocytes, monocytes, eosinophils, and basophils to vascular endothelium. VCAM-1 is an endothelial ligand for VLA-4 (Very Late Antigen-4 or α4β1) of the β1 subfamily of integrins, and for integrin α4β7. VCAM-1 expression has also been observed in other cell types. It has also been shown to interact with EZR and Moesin. Other roles proposed for VCAM-1 include the regulation of osteoclastogenesis via a cell-to-cell contact mechanism and the induction of sickle cell adherence to vascular endothelial cells during hypoxemia. It also functions in leukocyte-endothelial cell signal transduction. Monitoring of sVCAM-1 levels in serum provides more detailed insights in several pathological situations such as atherosclerosis and rheumatoid arthritis. |
