MULTI SCIENCES
Mouse Total MMP-3 ELISA Kit
Mouse Total MMP-3 ELISA Kit
SKU:EK2M03
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK2M03-96 |
| CatNum | EK2M03-96 |
| Product Name | Mouse Total MMP-3 ELISA Kit |
| Customs Name | Mouse Total MMP-3 ELISA Kit |
| Product Spec | 96T |
| SalePrice | 3200 |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Mouse |
| Modality | NoData |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sensitivity | 15.99 pg/ml |
| Standard Curve Range | 46.88 - 3000 pg/ml |
| Spike Recovery Range | 75 %-95 % |
| Mean Spike Recovery | 88% |
| CV of Intra plate | 2.9 % - 3.6 % |
| CV of Inter plate | 3.4 % - 4.8 % |
| Components |
96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against mouse total MMP-3 Mouse Total MMP-3 Standard, lyophilized Total MMP-3 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate Stop Solution Washing Buffer (20×) Adhesive Films |
| PRINCIPLE OF THE ASSAY | Mouse Total MMP-3 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse total MMP-3 in the samples. An antibody specific for mouse total MMP-3 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for total MMP-3, and total MMP-3 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of total MMP-3 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader. |
| Describtion | Matrix metalloproteinase-3 (MMP-3), also known as stromelysin-1, is an enzyme that in humans is encoded by the MMP3 gene. Expression of MMP3 is primarily regulated at the level of transcription, where the promoter of the gene responds to various stimuli, including growth factors, cytokines, tumor promoters, and oncogene products. The MMP-3 enzyme degrades collagen types II, III, IV, IX, and X, proteoglycans, fibronectin, laminin, and elastin. In addition, MMP-3 can also activate other MMPs such as MMP-1, MMP-7, and MMP-9, rendering MMP-3 crucial in connective tissue remodeling. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. In a study conducted using MMP-3 wild type and knockout mice, MMP-3 was shown to increase blood-brain barrier permeability after traumatic injury. Nuclear MMP-3 functions as a transcription factor and a proteinase. |
