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Mouse Total MMP-3 ELISA Kit

Mouse Total MMP-3 ELISA Kit


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Mouse Total MMP-3 ELISA Kit

Factory Name MultiSciences
Product Code 70-EK2M03-96
CatNum EK2M03-96
Product Name Mouse Total MMP-3 ELISA Kit
Customs Name Mouse Total MMP-3 ELISA Kit
Product Spec 96T
SalePrice 3200
Type ELISA Kit
Application ELISA
Reactivity Mouse
Modality NoData
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sensitivity 15.99 pg/ml
Standard Curve Range 46.88 - 3000 pg/ml
Spike Recovery Range 75 %-95 %
Mean Spike Recovery 88%
CV of Intra plate 2.9 % - 3.6 %
CV of Inter plate 3.4 % - 4.8 %

96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against mouse total MMP-3

Mouse Total MMP-3 Standard, lyophilized

Total MMP-3 Detect Antibody

Standard Diluent


Assay Buffer (10×)


Stop Solution

Washing Buffer (20×)

Adhesive Films


Mouse Total MMP-3 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse total MMP-3 in the samples. An antibody specific for mouse total MMP-3 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for total MMP-3, and total MMP-3 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of total MMP-3 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.


Matrix metalloproteinase-3 (MMP-3), also known as stromelysin-1, is an enzyme that in humans is encoded by the MMP3 gene. Expression of MMP3 is primarily regulated at the level of transcription, where the promoter of the gene responds to various stimuli, including growth factors, cytokines, tumor promoters, and oncogene products. The MMP-3 enzyme degrades collagen types II, III, IV, IX, and X, proteoglycans, fibronectin, laminin, and elastin. In addition, MMP-3 can also activate other MMPs such as MMP-1, MMP-7, and MMP-9, rendering MMP-3 crucial in connective tissue remodeling. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. In a study conducted using MMP-3 wild type and knockout mice, MMP-3 was shown to increase blood-brain barrier permeability after traumatic injury. Nuclear MMP-3 functions as a transcription factor and a proteinase.