96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against mouse TRAIL

Mouse TRAIL Standard, lyophilized

TRAIL Detect Antibody

Standard Diluent

Streptavidin-HRP                                                                                           

Assay Buffer (10×)

Substrate

Stop Solution

Washing Buffer (20×)

Adhesive Films

Mouse TRAIL/TNFSF10 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse TRAIL in the samples. An antibody specific for mouse TRAIL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and TRAIL present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for TRAIL is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of TRAIL bound in the initial step. The color development is stopped and the intensity of the color is measured.

TNF-related apoptosis-inducing ligand (TRAIL) also known as CD253 or TNFSF10, is a cytokine that is produced and secreted by most normal tissue cells. TRAIL binds to the death receptors DR4 (TRAIL-RI) and DR5 (TRAIL-RII). The process of apoptosis is caspase-8-dependent. Caspase-8 activates downstream effector caspases including procaspase-3, -6, and -7, leading to activation of specific kinases. TRAIL also binds the receptors DcR1 and DcR2, which do not contain a cytoplasmic domain (DcR1) or contain a truncated death domain (DcR2). DcR1 functions as a TRAIL-neutralizing decoy-receptor. The cytoplasmic domain of DcR2 is functional and activates NF-κB. In cells expressing DcR2, TRAIL binding therefore activates NF-κB, leading to transcription of genes known to antagonize the death signaling pathway and/or to promote inflammation.