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Rat GM-CSF ELISA for Protein Quantification

Rat GM-CSF ELISA for Protein Quantification


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Product Details

Rat GM-CSF ELISA for Protein Quantification

Brand MultiSciences
CatNum 70-EK363
Product Name Rat GM-CSF ELISA Kit
Customs Name Rat GM-CSF ELISA Kit
Application ELISA
Reactivity Rat
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 10 μl
Sensitivity 1.84 pg/ml
Standard Curve Range 31.25 - 2000 pg/ml
Spike Recovery Range 82 % - 128 %
Mean Spike Recovery 1.02
CV of Intra plate 3.0 % - 5.0 %
CV of Inter plate 2.4 % - 8.2 %
Release Date 2015/11/26
Components 96-well polystyrene microplate coated with a monoclonal antibody against GM-CSF
Rat GM-CSF Standard, lyophilized
GM-CSF Detect Antibody
Standard Diluent
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat GM-CSF. The Rat GM-CSF ELISA is for research use only. Not for use in therapeutic procedures.

Granulocyte - macrophage colony stimulating factor (GM-CSF) is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils and basophils) and monocytes. GM-CSF signals via signal transducer and activator of transcription, STAT5. In macrophages, it has also been shown to signal via STAT3. Although GM-CSF was initially characterized by its ability to stimulate neutrophil, monocyte / macrophage and eosinophil colony formation, it has since been shown that it has additional activities on hematopoietic, as well as non-hematopoietic lineage cells.
Increased levels of GM-CSF have been reported in the serum of some cancer patients, including patients with lung cancer and patients with acute myelogenous leukemia. GM-CSF has also been reported to be increased in the peripheral blood of bronchial asthma patients and in extracts of psoriatic scales.