MULTI SCIENCES
Rat GM-CSF ELISA for Protein Quantification
Rat GM-CSF ELISA for Protein Quantification
SKU:EK363
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK363 |
| Product Name | Rat GM-CSF ELISA Kit |
| Customs Name | Rat GM-CSF ELISA Kit |
| Application | ELISA |
| Reactivity | Rat |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 10 μl |
| Sensitivity | 1.84 pg/ml |
| Standard Curve Range | 31.25 - 2000 pg/ml |
| Spike Recovery Range | 82 % - 128 % |
| Mean Spike Recovery | 1.02 |
| CV of Intra plate | 3.0 % - 5.0 % |
| CV of Inter plate | 2.4 % - 8.2 % |
| Release Date | 2015/11/26 |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against GM-CSF Rat GM-CSF Standard, lyophilized GM-CSF Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion |
This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat GM-CSF. The Rat GM-CSF ELISA is for research use only. Not for use in therapeutic procedures. Granulocyte - macrophage colony stimulating factor (GM-CSF) is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils and basophils) and monocytes. GM-CSF signals via signal transducer and activator of transcription, STAT5. In macrophages, it has also been shown to signal via STAT3. Although GM-CSF was initially characterized by its ability to stimulate neutrophil, monocyte / macrophage and eosinophil colony formation, it has since been shown that it has additional activities on hematopoietic, as well as non-hematopoietic lineage cells.Increased levels of GM-CSF have been reported in the serum of some cancer patients, including patients with lung cancer and patients with acute myelogenous leukemia. GM-CSF has also been reported to be increased in the peripheral blood of bronchial asthma patients and in extracts of psoriatic scales. |
