|Product Name||Rat IFN-gamma ELISA Kit|
|Customs Name||Rat IFN-gamma ELISA Kit|
|Assay Type||Sandwich ELISA|
|Suitable Sample Type||serum, plasma, cell culture supernates|
|Format||96-well strip plate|
|Storage||4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)|
|Sample Volume||20 μl|
|Standard Curve Range||31.25 - 2000 pg/ml|
|Spike Recovery Range||87 % - 112 %|
|Mean Spike Recovery||0.99|
|CV of Intra plate||4.1 % - 6.0 %|
|CV of Inter plate||5.4 % - 6.6 %|
|Components||96-well polystyrene microplate coatedwithamonoclonalantibodyagainstIFN-γ
|Describtion||This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat IFN-γ. The Rat IFN-γ ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Interferon gamma (IFN-γ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. IFN-γ, or type II interferon, is a cytokine that is critical forinnate and adaptive immunity against viral, some bacterial and protozoal infections. IFN-γ is an important activator of macrophages and inducer of Class II major histocompatibility complex (MHC) molecule expression. Aberrant IFN-γ expression is associated with a number of autoinflammatory and autoimmunediseases. The importance of IFN-γ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFN-γ is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 Th1 and CD8 cytotoxic T lymphocyte (CTL) effector T cells onceantigen- specific immunity develops.
The role of IFN-γ as a disease marker has been demonstrated for a number of different pathological situations including infections, autoimmune diseases, transplant rejection, allergy and diabetes.