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Rat IgG2a Enzyme Immunoassay Kit

Rat IgG2a Enzyme Immunoassay Kit


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Rat IgG2a  Enzyme Immunoassay Kit

CatNum 70-EK373
Product Name Rat IgG2a ELISA Kit
Customs Name Rat IgG2a ELISA Kit
Application ELISA
Reactivity Rat
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 20 μl (prediluted)
Sensitivity 22.52 pg/ml
Standard Curve Range 0.78 - 50 ng/ml
Spike Recovery Range 80%-110%
Mean Spike Recovery 95%
CV of Intra plate 3.5% - 5.0%
CV of Inter plate 3.8% - 4.3%
Components 96-well polystyrene microplate coated with a monoclonal antibody against IgG2a
Rat IgG2a Standard, lyophilized
IgG2a Detect Antibody
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
Describtion This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat IgG2a. The Rat IgG2a ELISA is for research use only. Not for use in therapeutic procedures.
Immunoglobulin G (IgG) is a type of antibody created and released by plasma B cells. IgG is the main type of antibody in humoral immunity found in blood and extracellular fluid allowing it to control infection of body tissues. By binding many kinds of pathogens such as viruses, bacteria, and fungi, IgG protects the body from infection. There are four IgG subclasses (IgG1, 2, 3, and 4) in humans, and four IgG subclasses (IgG1, 2a, 2b, 3) in mice and rats. The measurement of IgG can be a diagnostic tool for certain conditions, such as autoimmune hepatitis, if indicated by certain symptoms. Clinically, measured IgG antibody levels are generally considered to be indicative of an individual's immune status to particular pathogens.