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Rat IL-17A ELISA Kit

Rat IL-17A ELISA Kit


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Product Details

Rat IL-17A ELISA Kit

Factory Name MultiSciences
Product Code 70-EK3172/2
Product Name Rat IL-17A ELISA Kit
Customs Name Rat IL-17A ELISA Kit
Product Spec 96 T
Type ELISA Kit
Application ELISA
Reactivity Rat
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 10 μl
Sensitivity 0.62 pg/ml
Standard Curve Range 3.13 - 200 pg/ml
Spike Recovery Range 94 % - 119 %
Mean Spike Recovery 103%
CV of Intra plate 3.9 % - 7.1 %
CV of Inter plate 3.0 % - 3.9 %

96-well polystyrene microplate coated with a monoclonal antibody against IL-17A

Human IL-17A Standard, lyophilized

IL-17A Detect Antibody

Standard Diluent


Amplification Reagent Concentrate

Amplification Diluent

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

washing Buffer (20×)

Plate Covers

Describtion This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for rat IL-17A has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for IL-17A are pipetted into the wells and IL-17A present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IL-17A bound in the initial step. The color development is stopped and the intensity of the color is measured.