MULTI SCIENCES
Rat IL-17A ELISA Kit
Rat IL-17A ELISA Kit
SKU:EK3172
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK3172/2 |
| Product Name | Rat IL-17A ELISA Kit |
| Customs Name | Rat IL-17A ELISA Kit |
| Product Spec | 96 T |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Rat |
| SDS | SDS - EK317 |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 10 μl |
| Sensitivity | 0.62 pg/ml |
| Standard Curve Range | 3.13 - 200 pg/ml |
| Spike Recovery Range | 94 % - 119 % |
| Mean Spike Recovery | 103% |
| CV of Intra plate | 3.9 % - 7.1 % |
| CV of Inter plate | 3.0 % - 3.9 % |
| Components |
96-well polystyrene microplate coated with a monoclonal antibody against IL-17A Human IL-17A Standard, lyophilized IL-17A Detect Antibody Standard Diluent Streptavidin-HRP Amplification Reagent Concentrate Amplification Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for rat IL-17A has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for IL-17A are pipetted into the wells and IL-17A present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IL-17A bound in the initial step. The color development is stopped and the intensity of the color is measured. |
