|Product Name||Rat IL-1beta ELISA Kit|
|Customs Name||Rat IL-1beta ELISA Kit|
|Assay Type||Sandwich ELISA|
|Suitable Sample Type||serum, plasma, cell culture supernates|
|Format||96-well strip plate|
|Storage||4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)|
|Sample Volume||20 μl|
|Standard Curve Range||31.25 - 2000 pg/ml|
|Spike Recovery Range||84 % - 110 %|
|Mean Spike Recovery||1.02|
|CV of Intra plate||5.0 % - 5.1 %|
|CV of Inter plate||5.5 % - 6.7 %|
|Components||96-well polystyrene microplate coated with a monoclonal antibody against IL-1β
Rat IL-1β Standard, lyophilized
IL-1β Detect Antibody
Assay Buffer (10×)
washing Buffer (20×)
|Describtion||This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat IL-1β. The Rat IL-1β ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Interleukin 1 (IL-1) is an extracellular peptide of 17 kDa that designates two proteins, IL-1α and IL-1β. IL-1β is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis.The interleukin-1 (IL-1) species represent an important family of biologically active mono nuclear cell-derived proteins which are involved in inflammatory reactions and in immune responses.
Increased production of IL-1β causes a number of different autoinflammatory syndromes, most notably the monogenic conditions referred to as CAPS, due to mutations in the inflammasome receptor NLRP3 which triggers processing of IL-1β.