MULTI SCIENCES
Rat IL-1beta Cytokine ELISA Kit
Rat IL-1beta Cytokine ELISA Kit
SKU:EK301B
Product Details
Brand | MultiSciences |
---|---|
CatNum | 70-EK301B |
Product Name | Rat IL-1beta ELISA Kit |
Customs Name | Rat IL-1beta ELISA Kit |
Application | ELISA |
Reactivity | Rat |
Assay Type | Sandwich ELISA |
Suitable Sample Type | serum, plasma, cell culture supernates |
Format | 96-well strip plate |
Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
Shipping Condition | 4℃ |
Sample Volume | 20 μl |
Sensitivity | 1.42 pg/ml |
Standard Curve Range | 31.25 - 2000 pg/ml |
Spike Recovery Range | 84 % - 110 % |
Mean Spike Recovery | 1.02 |
CV of Intra plate | 5.0 % - 5.1 % |
CV of Inter plate | 5.5 % - 6.7 % |
Release Date | 2016/8/20 |
Components | 96-well polystyrene microplate coated with a monoclonal antibody against IL-1β Rat IL-1β Standard, lyophilized IL-1β Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat IL-1β. The Rat IL-1β ELISA is for research use only. Not for diagnostic or therapeutic procedures. Interleukin 1 (IL-1) is an extracellular peptide of 17 kDa that designates two proteins, IL-1α and IL-1β. IL-1β is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis.The interleukin-1 (IL-1) species represent an important family of biologically active mono nuclear cell-derived proteins which are involved in inflammatory reactions and in immune responses. Increased production of IL-1β causes a number of different autoinflammatory syndromes, most notably the monogenic conditions referred to as CAPS, due to mutations in the inflammasome receptor NLRP3 which triggers processing of IL-1β. |
