MULTI SCIENCES
Rat IL-1β Sandwich High Sensitivity ELISA Kit
Rat IL-1β Sandwich High Sensitivity ELISA Kit
SKU:EK301BHS
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK301BHS |
| CatNum | EK301BHS |
| Product Name | Rat IL-1β High Sensitivity ELISA Kit |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Rat |
| SDS | SDS - EK301BHS |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 10 μl |
| Sensitivity | 0.71 pg/ml |
| Standard Curve Range | 7.81 - 500 pg/ml |
| Spike Recovery Range | 89 % - 127 % |
| Mean Spike Recovery | 106% |
| CV of Intra plate | 3.8 % - 5.2 % |
| CV of Inter plate | 4.9 % - 5.6 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against IL-1β Rat IL-1β Standard, lyophilized IL-1β Detect Antibody Standard Diluent Streptavidin-HRP Amplification Reagent Concentrate Amplification Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat IL-1β. The Rat IL-1βHS ELISA is for research use only. Not for diagnostic or therapeutic procedures. Interleukin-1 (IL-1) is an extracellular peptide of 17 kDa that designates two proteins, IL-1α and IL-1β. IL-1β is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The interleukin-1 (IL-1) species represent an important family of biologically active mono nuclear cell-derived proteins which are involved in inflammatory reactions and in immune responses. Increased production of IL-1β causes a number of different autoinflammatory syndromes, most notably the monogenic conditions referred to as CAPS, due to mutations in the inflammasome receptor NLRP3 which triggers processing of IL-1β. |
