MULTI SCIENCES
Rat IL-2 High Sensitivity ELISA Kit
Rat IL-2 High Sensitivity ELISA Kit
SKU:EK302HS
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK302HS-96 |
| CatNum | EK302HS-96 |
| Product Name | Rat IL-2 High Sensitivity ELISA Kit |
| Customs Name | Rat IL-2 High Sensitivity ELISA Kit |
| Product Spec | 96 T |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Rat |
| SDS | SDS - EK302HS |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 20 μl |
| Sensitivity | 0.71 pg/ml |
| Standard Curve Range | 12.5 - 800 pg/ml |
| Spike Recovery Range | 95 % - 119 % |
| Mean Spike Recovery | 103% |
| CV of Intra plate | 4.0% - 4.4% |
| CV of Inter plate | 3.9% - 4.8% |
| Components |
96-well polystyrene microplate coated with a monoclonal antibody against IL-2 Rat IL-2 Standard, lyophilized IL-2 Detect Antibody Standard Diluent Streptavidin-HRP Amplification Reagent Concentrate Amplification Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Cover |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for rat IL-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and IL-2 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for IL-2 is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, amplification reagent is added to the wells. Following incubation any unbound substances is removed during a wash step and streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
