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Rat IL-4 ELISA for Protein Quantification

Rat IL-4 ELISA for Protein Quantification


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Product Details

Rat IL-4 ELISA for Protein Quantification

Brand MultiSciences
CatNum 70-EK304
Product Name Rat IL-4 ELISA Kit
Customs Name Rat IL-4 ELISA Kit
Application ELISA
Reactivity Rat
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 20 μl
Sensitivity 0.26 pg/ml
Standard Curve Range 15.63 - 1000 pg/ml
Spike Recovery Range 86 % - 106 %
Mean Spike Recovery 0.99
CV of Intra plate 2.1 % - 2.7 %
CV of Inter plate 3.1 % - 4.3 %
Release Date 2016/8/20
Components 96-well polystyrene microplate coated with a monoclonal antibody against IL-4
Rat IL-4 Standard, lyophilized
IL-4 Detect Antibody
Standard Diluent
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
Describtion This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat IL-4. The Rat IL-4 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Interleukin 4 (IL-4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. The cell that initially produces IL-4, thus inducing Th0 differentiation, has not been identified, but recent studies suggest that basophils may be the effector cell. It is closely related and has functions similar to IL-13.

IL-4 also has been shown to drive mitogenesis, dedifferentiation,and metastasis in rhabdomyosarcoma. IL-4, along with other Th2 cytokines, is involved in the airway inflammation observed in the lungs of patients with allergic asthma.