|Product Name||Rat IL-4 High Sensitivity ELISA Kit|
|SDS||SDS - EK304HS|
|Assay Type||Sandwich ELISA|
|Suitable Sample Type||serum, plasma, cell culture supernates|
|Format||96-well strip plate|
|Storage||4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)|
|Sample Volume||20 μl|
|Standard Curve Range||3.13 - 200 pg/ml|
|Spike Recovery Range||81 % - 101 %|
|Mean Spike Recovery||90%|
|CV of Intra plate||5.0 % - 6.8 %|
|CV of Inter plate||2.7% - 7.5 %|
|Components||96-well polystyrene microplate coated with a monoclonal antibody against IL-4
Rat IL-4 Standard, lyophilized
IL-4 Detect Antibody
Amplification Reagent Concentrate
Assay Buffer (10×)
washing Buffer (20×)
|Describtion||This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat IL-4. The Rat IL-4HS ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Interleukin 4 (IL-4) is an approximately 18 - 20 kDa monomeric cytokine, which induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. The cell that initially produces IL-4, thus inducing Th0 differentiation, has not been identified, but recent studies suggest that basophils may be the effector cell. It is closely related and has functions similar to IL-13.
IL-4 also has been shown to drive mitogenesis, dedifferentiation and metastasis in rhabdomyosarcoma. IL-4, along with other Th2 cytokines, is involved in the airway inflammation observed in the lungs of patients with allergic asthma.