MULTI SCIENCES
Rat IL-4 High Sensitivity ELISA Assay Kit
Rat IL-4 High Sensitivity ELISA Assay Kit
SKU:EK304HS
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK304HS |
| CatNum | EK304HS |
| Product Name | Rat IL-4 High Sensitivity ELISA Kit |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Rat |
| SDS | SDS - EK304HS |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 20 μl |
| Sensitivity | 0.2 pg/ml |
| Standard Curve Range | 3.13 - 200 pg/ml |
| Spike Recovery Range | 81 % - 101 % |
| Mean Spike Recovery | 90% |
| CV of Intra plate | 5.0 % - 6.8 % |
| CV of Inter plate | 2.7% - 7.5 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against IL-4 Rat IL-4 Standard, lyophilized IL-4 Detect Antibody Standard Diluent Streptavidin-HRP Amplification Reagent Concentrate Amplification Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat IL-4. The Rat IL-4HS ELISA is for research use only. Not for diagnostic or therapeutic procedures. Interleukin 4 (IL-4) is an approximately 18 - 20 kDa monomeric cytokine, which induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. The cell that initially produces IL-4, thus inducing Th0 differentiation, has not been identified, but recent studies suggest that basophils may be the effector cell. It is closely related and has functions similar to IL-13. IL-4 also has been shown to drive mitogenesis, dedifferentiation and metastasis in rhabdomyosarcoma. IL-4, along with other Th2 cytokines, is involved in the airway inflammation observed in the lungs of patients with allergic asthma. |
