MULTI SCIENCES
Rat IL-6 ELISA Kit Plate
Rat IL-6 ELISA Kit Plate
SKU:EK306
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK306 |
| Product Name | Rat IL-6 ELISA Kit |
| Customs Name | Rat IL-6 ELISA Kit |
| Application | ELISA |
| Reactivity | Rat |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 20 μl |
| Sensitivity | 2.57 pg/ml |
| Standard Curve Range | 62.50 - 4000 pg/ml |
| Spike Recovery Range | 92 % - 104 % |
| Mean Spike Recovery | 0.97 |
| CV of Intra plate | 4.2 % - 7.7 % |
| CV of Inter plate | 6.3 % - 9.1 % |
| Release Date | 2016/8/20 |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against IL-6 Rat IL-6 Standard, lyophilized IL-6 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat IL-6. The Rat IL-6 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Interleukin 6 (IL-6) is an interleukin that acts as both a pro - inflammatory cytokine and an anti - inflammatory myokine. Rat IL-6 cDNA encodes a 211 amino acid (aa) residue precursor polypeptide with a hydrophobic signal peptide that is cleaved to generate the 187 aa residue mature protein. At the protein sequence level, there is approximately 39% identity between rat and human, and 87% identity between mouse and rat IL-6. IL-6 is secreted by T cells and macrophages to stimulate immune response, e.g. during infection and after trauma, especially burns or other tissue damage leading to inflammation. IL-6 also plays a role in fighting infection, as IL-6 has been shown in mice to be required for resistance against bacteriumStreptococcus pneumoniae. IL-6 stimulates the inflammatory and auto - immune processes in many diseases such as diabetes, atherosclerosis, depression, Alzheimer's Disease, systemic lupus erythematosus, multiple myeloma, prostate cancer, Beh?et's disease, and rheumatoid arthritis. Advanced/ metastatic cancer patients have higher levels of IL-6 in their blood. |
