MULTI SCIENCES
Rat TIMP-1 ELISA Kit
Rat TIMP-1 ELISA Kit
SKU:EK3138
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK3138-96 |
| CatNum | EK3138-96 |
| Product Name | Rat TIMP-1 ELISA Kit |
| Customs Name | Rat TIMP-1 ELISA Kit |
| Product Spec | 96T |
| SalePrice | 3200 |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Rat |
| SDS | SDS - EK280 |
| Modality | NoData |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sensitivity | 3.66 pg/ml |
| Standard Curve Range | 31.25 - 2000 pg/ml |
| Spike Recovery Range | 88 %-114 % |
| Mean Spike Recovery | 101% |
| CV of Intra plate | 3.8 % - 4.4 % |
| CV of Inter plate | 3.4 % - 4.8 % |
| Components |
96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against rat TIMP-1 Rat TIMP-1 Standard, lyophilized TIMP-1 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate Stop Solution Washing Buffer (20×) Adhesive Films |
| PRINCIPLE OF THE ASSAY | Rat TIMP-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of rat TIMP-1 in the samples. An antibody specific for rat TIMP-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for TIMP-1, and TIMP-1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of TIMP-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader. |
| Describtion |
Tissue inhibitor of matrix metalloprotease-1 (TIMP-1) is a glycoprotein that is expressed from the several tissues of organisms. This protein is a member of the TIMP family. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. Study suggests that this gene inactivation is polymorphic in human females. In adrenocortical cells the trophic hormone ACTH induces expression of TIMP-1 and the increase in TIMP expression is also associated with decreased collagenase activity. In addition, increased expression of TIMP-1 has been found to be associated with worse prognosis of various tumors, such as laryngeal carcinoma or melanoma. |
