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Rat TNF-α High Sensitivity Antibody ELISA Kit

Rat TNF-α High Sensitivity Antibody ELISA Kit


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Rat TNF-α High Sensitivity Antibody ELISA Kit

Factory Name MultiSciences
Product Code 70-EK382HS
Cat Num EK382HS
Product Name Rat TNF-α High Sensitivity ELISA Kit
Customs Name Rat TNF-α High Sensitivity ELISA Kit
SalePrice 3200
Application ELISA
Reactivity Rat
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 20 μl
Sensitivity 1.73pg/ml
Standard Curve Range 7.81 - 500 pg/ml
Spike Recovery Range 92%-127%
Mean Spike Recovery 112%
CV of Intra plate 4.2 % - 4.8%
CV of Inter plate 3.7 % - 10.1 %

96-well polystyrene microplate coated with a monoclonal antibody against TNF-α

Rat TNF-α Standard, lyophilized

TNF-α Detect Antibody

Standard Diluent


Amplification Reagent Concentrate

Amplification Diluent

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

washing Buffer (20×)

Plate Covers


This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat TNF-α. The Rat TNF-α ELISA is for research use only. Not for diagnostic or therapeutic procedures

.Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF1A, is an adipokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It functions as a multipotent modulator of immune response and further acts as a potent pyrogen. TNF-α circulates throughout the body responding to stimuli (infectious agents or tissue injury), activating neutrophils, altering the properties of vascular endothelial cells, regulating metabolic activities of other tissues, as well as exhibiting tumoricidal activity by inducing localized blood clotting.

TNF-α may play a significant role in the pathogenesis of inflammatory disease of the joints and other tissues. Recently, a growing body of information has pointed to a role for TNF-α in the pathogenesis of AIDS. Measurement of TNF-α levels has also been shown to be useful in transplant research.