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Rat VEGF High Sensitivity ELISA Kit

Rat VEGF High Sensitivity ELISA Kit


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Product Details

Rat VEGF High Sensitivity ELISA Kit

Factory Name


Product Code 70-EK383HS-96


Product Name

Rat VEGF High Sensitivity ELISA Kit

Product Spec 96T
Sale Price 3200
Type ELISA Kit





Assay Type

Sandwich ELISA

Suitable Sample Type

serum, plasma, cell culture supernates


96-well strip plate


4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)

Shipping Condition



0.34 pg/ml

Standard Curve Range

3.12- 200 pg/ml

Spike Recovery Range

91 %-111 %

Mean Spike Recovery


CV of Intra plate

4.2 % - 4.8 %

CV of Inter plate

4.6 % - 5 %


96-well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody against rat VEGF

Rat VEGF HS Standard, lyophilized

IL-12p70 HS Detect Antibody

Standard Diluent


Assay Buffer (10×)


Stop Solution

Washing Buffer (20×)

Plate Covers                                                                                   

PRINCIPLE OF THE ASSAY Rat VEGF High Sensitivity ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of rat VEGF in the samples. A monoclonal antibody specific for rat VEGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and VEGF present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for VEGF is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, amplification reagent is added to the wells. Following incubation any unbound substances is removed during a wash step and streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

Vascular endothelial growth factor (VEGF) is a sub-family of growth factors, to be specific, the platelet-derived growth factor family of cystine-knot growth factors. They are important signaling proteins involved in both vasculogenesis and angiogenesis. VEGF is a signal protein produced by cells that simulates vasculogenesis and angiogenesis. It is part of the system that restores the oxygen supply to tissues when blood circulation is inadequate. VEGF is best known for its role in vasculogenesis. During embryogenesis, VEGF regulates the proliferation, migration, and survival of endothelial cells, thus regulating blood vessel density and size, but playing no role in determining vascular patterns. Serum concentration of VEGF is high in bronchial asthma and diabetes mellitus. Overexpression of VEGF can cause vascular disease in the retina of the eye and other parts of the body. Drugs such as bevacizumab and Ranibizumab can inhibit VEGF and control or slow those diseases.