EasyGo!™Human TNF-α One-Step ELISA Kit

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TARGETSTNF
SPECIESHuman
Sample TypeSerum, plasma, DMEM cell culture supernatant
Sample VolumeSerum or plasma: 10μL; DMEM cell culture supernatant: 33μL
Sensitivity1.77 pg/mL
Range3.13 pg/mL – 200 pg/mL
Assay Time1.5 h
Recovery100% – 122%
Average Recovery1.08
Sample TypeSerum, plasma, DMEM cell culture supernatant
Sample VolumeSerum or plasma: 10μL; DMEM cell culture supernatant: 33μL
Sensitivity1.77 pg/mL
Range3.13 pg/mL – 200 pg/mL
Assay Time1.5 h
Recovery100% – 122%
Average Recovery1.08
Intra Precision4.2% – 5.4%
Inter-Precision5.5% – 6.2%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TNF-α monoclonal antibody
Human TNF-α freeze-dried standard
TNF-α detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for human TNF-α has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for TNF-α (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted intothe wells, then TNF-α present is bound by the immobilized antibody and detect antibody, of whichthe latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solutionreacts with HRP and color develops in proportion to the amount of TNF-α bound by the immobilized antibody. The color development is stopped and the intensity of the color is measuredby microplate reader.

Targets

TNF

TNF Target Infomation Overview

  • Target Symbol: TNF, tumor necrosis factor
  • Gene Groups: Tumor necrosis factor superfamily
  • Alias: TNFSF2; DIF; TNF-alpha
  • Previous Names: TNFA
  • Alias Names: TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF, tumor necrosis factor Target Infomation by Species

HumanMouseRat

Human TNF Target Information

  • Target Symbol: TNF, tumor necrosis factor
  • Alias:
    • APC1 protein
    • cachectin
    • DIF
    • TNF superfamily, member 2
    • TNF-a
    • TNF-alpha
    • TNF, macrophage-derived
    • TNF, monocyte-derived
    • TNFA
    • TNFSF2
    • TNLG1F
    • tumor necrosis factor alpha
    • tumor necrosis factor ligand 1F
    • tumor necrosis factor ligand superfamily member 2
    • tumor necrosis factor-alpha
  • NCBI_Gene: 7124
  • UniProtKB: P01375

Human TNF Predicted Functions

Enables several functions, including identical protein binding activity; protease binding activity; and signaling receptor binding activity. Involved in several processes, including negative regulation of macromolecule metabolic process; positive regulation of macromolecule metabolic process; and regulation of apoptotic process. Acts upstream of or within several processes, including regulation of cellular protein metabolic process; regulation of gene expression; and regulation of signal transduction. Located in cell surface; extracellular space; and membrane raft. Is integral component of plasma membrane. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Biomarker of several diseases, including anemia (multiple); autoimmune disease (multiple); eye disease (multiple); kidney disease (multiple); and lung disease (multiple).

Mouse Tnf Target Information

Mouse Tnf Predicted Functions

Enables cytokine activity; protease binding activity; and tumor necrosis factor receptor binding activity. Involved in several processes, including endothelial cell apoptotic process; positive regulation of cell communication; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including apoptotic signaling pathway; positive regulation of intracellular signal transduction; and regulation of gene expression. Located in several cellular components, including external side of plasma membrane; phagocytic cup; and recycling endosome. Is expressed in several structures, including alimentary system; brain; integumental system; lung; and reproductive system. Used to study several diseases, including autoimmune disease (multiple); congestive heart failure; heart valve disease (multiple); idiopathic pulmonary fibrosis; and spondyloarthropathy. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).

Rat Tnf Target Information

  • Target Symbol: Tnf, tumor necrosis factor
  • Alias:
    • cachectin
    • LOC103694380
    • MGC124630
    • RATTNF
    • TNF-a
    • TNF-alpha
    • Tnfa
    • tumor necrosis factor (TNF superfamily, member 2)
    • tumor necrosis factor alpha
    • tumor necrosis factor ligand superfamily member 2
    • tumor necrosis factor superfamily member 2
    • tumor necrosis factor superfamily, member 2
    • tumor necrosis factor-alpha
    • tumor necrosis factor-like
  • NCBI_Gene: 24835
  • UniProtKB: P16599

Rat Tnf Predicted Functions

Enables tumor necrosis factor receptor binding activity. Involved in several processes, including positive regulation of cell communication; positive regulation of macromolecule metabolic process; and positive regulation of neuron death. Located in several cellular components, including external side of plasma membrane; extracellular space; and neuronal cell body. Used to study several diseases, including cerebrovascular disease (multiple); liver disease (multiple); peptic esophagitis; perinatal necrotizing enterocolitis; and periventricular leukomalacia. Biomarker of several diseases, including brain disease (multiple); congestive heart failure (multiple); inflammatory bowel disease (multiple); lung disease (multiple); and non-alcoholic fatty liver disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).

TNF Target News

    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Eye Diseases (Glaucoma & Cataract)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Osteoporosis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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