Human DLL1 ELISA KIT

$350.00$450.00

SPECIES Human
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 100 μL (diluent)
Sensitivity 9.49 pg/mL
Range 23.44 pg/mL – 1500 pg/mL
Assay Time 3.5 h
Recovery 83% – 107%
Average Recovery 0.95
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 100 μL (diluent)
Sensitivity 9.49 pg/mL
Range 23.44 pg/mL – 1500 pg/mL
Assay Time 3.5 h
Recovery 83% – 107%
Average Recovery 0.95
Intra Precision 7.0 % – 7.9%
Inter-Precision 5.8 % – 7.2%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with mouse anti-human DLL1 capture antibody
Human DLL1 freeze-dried standard
Human DLL1 detect Antibody
Streptavidin-HRP
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Standard Diluent
Assay Principle This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human DLL1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, DLL1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of DLL1 in the samples.加入终止液终止反应,在450 nm 波长(参考波长570 – 630 nm)测定吸光度值。

 

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