Name: Human Apolipoprotein B/Apo B ELISA Kit
SKU: EK1203
Availability: InStock

EK1203

Human Apolipoprotein B/Apo B ELISA Kit

$245.00 – $350.00

Related Target	APOB
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	74.26 pg/mL
Array Range	0.31 ng/mL – 20 ng/mL
Assay Time	3.5 h
Recovery	88% – 108%
Average Recovery	98%

ELISA Kit Detail Information

Related Target	APOB
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	74.26 pg/mL
Array Range	0.31 ng/mL – 20 ng/mL
Assay Time	3.5 h
Recovery	88% – 108%
Average Recovery	98%
Intra Precision	5.0% – 6.0%
Inter Precision	2.3% - 6.1%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Apolipoprotein B/Apo B monoclonal antibody Human Apolipoprotein B/Apo B freeze-dried standard Apolipoprotein B/Apo B detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Apo B antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Apo B present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Apo B in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

APOB

APOB Target Infomation Overview

Target Symbol: APOB, apolipoprotein B
Gene Groups: Apolipoproteins
Alias Names: apolipoprotein B (including Ag(x) antigen)

APOB, apolipoprotein B Target Infomation by Species

Human
Mouse
Rat

Human APOB Target Information

Target Symbol: APOB, apolipoprotein B
Alias:
- apo B-100
- apoB-100
- apoB-48
- apolipoprotein B (including Ag(x) antigen)
- apolipoprotein B-100
- apolipoprotein B48
- FCHL2
- FLDB
- LDLCQ4
- mutant Apo B 100
NCBI_Gene: 338
UniProtKB: P04114

Human APOB Predicted Functions

Enables several functions, including cholesterol transfer activity; heparin binding activity; and lipase binding activity. Involved in several processes, including cholesterol homeostasis; low-density lipoprotein particle clearance; and low-density lipoprotein particle remodeling. Located in cytosol; endoplasmic reticulum exit site; and neuronal cell body. Part of low-density lipoprotein particle; mature chylomicron; and triglyceride-rich plasma lipoprotein particle. Implicated in several diseases, including artery disease (multiple); biliary tract cancer (multiple); familial hyperlipidemia (multiple); hypobetalipoproteinemia (multiple); and sickle cell anemia. Biomarker of several diseases, including common bile duct neoplasm; diabetes mellitus (multiple); familial combined hyperlipidemia; hypobetalipoproteinemia; and obesity.

Mouse Apob Target Information

Target Symbol: Apob, apolipoprotein B
Alias:
- AI315052
- apob-100
- apob-48
- expressed sequence AI315052
NCBI_Gene: 238055

Mouse Apob Predicted Functions

Predicted to enable several functions, including cholesterol transfer activity; heparin binding activity; and lipase binding activity. Acts upstream of or within several processes, including cholesterol efflux; lipoprotein metabolic process; and triglyceride metabolic process. Located in endoplasmic reticulum. Is expressed in several structures, including adrenal gland; brain; extraembryonic component; genitourinary system; and gut. Used to study familial hypobetalipoproteinemia 2. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); biliary tract cancer (multiple); familial hyperlipidemia (multiple); hypobetalipoproteinemia (multiple); and sickle cell anemia. Orthologous to human APOB (apolipoprotein B).

Rat Apob Target Information

Target Symbol: Apob, apolipoprotein B
Alias:
- Aa1064
- Ac1-060
- Apo B-100
- ApoB-100
- ApoB-48
- apolipoprotein B (including Ag(x) antigen)
- apolipoprotein B PI
- apolipoprotein B-100
- apolipoprotein B-48
NCBI_Gene: 54225

Rat Apob Predicted Functions

Predicted to enable several functions, including cholesterol transfer activity; heparin binding activity; and lipase binding activity. Involved in several processes, including cellular response to tumor necrosis factor; response to estradiol; and triglyceride catabolic process. Located in several cellular components, including extracellular space; vesicle lumen; and vesicle membrane. Part of high-density lipoprotein particle; low-density lipoprotein particle; and very-low-density lipoprotein particle. Biomarker of fatty liver disease and nephrotic syndrome. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); biliary tract cancer (multiple); familial hyperlipidemia (multiple); hypobetalipoproteinemia (multiple); and sickle cell anemia. Orthologous to human APOB (apolipoprotein B).

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