Name: Human Angiopoietin-1/ANG-1 ELISA Kit
SKU: EK1122
Availability: InStock

EK1122

Human Angiopoietin-1/ANG-1 ELISA Kit

$245.00 – $350.00

Related Target	ANGPT1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	1.30 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	80% – 110%
Average Recovery	94%

ELISA Kit Detail Information

Related Target	ANGPT1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	1.30 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	80% – 110%
Average Recovery	94%
Intra Precision	3.3% – 5.4%
Inter Precision	5.0% – 8.2%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Angipoietin-1/ANG-1 monoclonal antibody Human Angipoietin-1/ANG-1 freeze-dried standard Angipoietin-1/ANG-1 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human ANG-1 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the ANG-1 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of ANG-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

ANGPT1

ANGPT1 Target Infomation Overview

Target Symbol: ANGPT1, angiopoietin 1
Gene Groups: Receptor ligands; Fibrinogen C domain containing
Alias: KIAA0003; Ang1

ANGPT1, angiopoietin 1 Target Infomation by Species

Human
Mouse
Rat

Human ANGPT1 Target Information

Target Symbol: ANGPT1, angiopoietin 1
Alias:
- AGP1
- AGPT
- ANG-1
- ANG1
- angiopoietin-1
- HAE5
NCBI_Gene: 284
UniProtKB: Q15389

Human ANGPT1 Predicted Functions

Enables receptor tyrosine kinase binding activity. Involved in several processes, including positive regulation of intracellular signal transduction; positive regulation of macromolecule metabolic process; and regulation of vascular permeability. Located in several cellular components, including extracellular space; membrane raft; and microvillus. Implicated in brain ischemia; diabetic retinopathy; and hypertension. Biomarker of carcinoma (multiple); hypertension; and ovarian cancer.

Mouse Angpt1 Target Information

Target Symbol: Angpt1, angiopoietin 1
Alias:
- 1110046O21Rik
- Ang-1
- ang1
- Angiopoietin-1
- RIKEN cDNA 1110046O21 gene
NCBI_Gene: 11600

Mouse Angpt1 Predicted Functions

Enables signaling receptor binding activity. Acts upstream of or within several processes, including blood vessel development; regulation of protein phosphorylation; and regulation of signal transduction. Predicted to be located in membrane raft; microvillus; and plasma membrane. Predicted to be active in collagen-containing extracellular matrix and extracellular space. Is expressed in several structures, including cardiovascular system; liver; lung; metanephros; and yolk sac. Human ortholog(s) of this gene implicated in brain ischemia; diabetic retinopathy; and hypertension. Orthologous to human ANGPT1 (angiopoietin 1).

Rat Angpt1 Target Information

Target Symbol: Angpt1, angiopoietin 1
Alias:
- Agpt
- Agpt1
- Ang-1
- angiopoietin
- angiopoietin-1
- LOC360422
NCBI_Gene: 89807
- UniprotKB: F1LSB2
- UniprotKB: O35460

Rat Angpt1 Predicted Functions

Enables identical protein binding activity. Involved in several processes, including animal organ development; response to estrogen; and response to vitamin B3. Located in extracellular space. Biomarker of gastric ulcer; in situ carcinoma; myocardial infarction; nephrosis; and pulmonary hypertension. Human ortholog(s) of this gene implicated in brain ischemia; diabetic retinopathy; and hypertension. Orthologous to human ANGPT1 (angiopoietin 1).

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