Human ACE2 ELISA Kit

$245.00 – $350.00

Related Target	ACE2
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	29.60 pg/mL
Array Range	0.31 ng/mL – 20 ng/mL
Assay Time	3.5 h
Recovery	98% – 121%
Average Recovery	108%

ELISA Kit Detail Information

Related Target	ACE2
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	29.60 pg/mL
Array Range	0.31 ng/mL – 20 ng/mL
Assay Time	3.5 h
Recovery	98% – 121%
Average Recovery	108%
Intra Precision	3.1% – 4.9%
Inter Precision	3.6% - 4.2%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-ACE2 monoclonal antibody Human ACE2 freeze-dried standard ACE2 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human ACE2 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the ACE2 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of ACE2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

ACE2

ACE2 Target Infomation Overview

Target Symbol: ACE2, angiotensin converting enzyme 2
Alias: ACEH
Alias Names: peptidyl-dipeptidase A; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin I converting enzyme 2

ACE2, angiotensin converting enzyme 2 Target Infomation by Species

Human
Mouse
Rat

Human ACE2 Target Information

Target Symbol: ACE2, angiotensin converting enzyme 2
Alias:
- ACE-related carboxypeptidase
- ACEH
- angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
- angiotensin I converting enzyme 2
- angiotensin-converting enzyme 2
- angiotensin-converting enzyme homolog
- angiotensin-converting enzyme-related carboxypeptidase
- DKFZp434A014
- metalloprotease MPROT15
- peptidyl-dipeptidase A
- truncated angiotensin converting enzyme 2
NCBI_Gene: 59272
UniProtKB: Q9BYF1

Human ACE2 Predicted Functions

Enables identical protein binding activity; peptidase activity; and virus receptor activity. Involved in several processes, including angiotensin-mediated drinking behavior; positive regulation of gap junction assembly; and regulation of transmembrane transport. Located in cell surface; extracellular space; and plasma membrane. Part of apical plasma membrane and brush border membrane. Implicated in several diseases, including avian influenza; cerebral malaria; hypertension (multiple); respiratory syncytial virus infectious disease; and severe acute respiratory syndrome. Biomarker of congestive heart failure; hypertrophic cardiomyopathy; and pancreatic ductal carcinoma.

Mouse Ace2 Target Information

Target Symbol: Ace2, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Alias:
- 2010305L05Rik
- RIKEN cDNA 2010305L05 gene
NCBI_Gene: 70008

Mouse Ace2 Predicted Functions

Predicted to enable identical protein binding activity; peptidase activity; and virus receptor activity. Involved in positive regulation of cardiac muscle contraction and tryptophan transport. Located in brush border membrane and cytoplasm. Is expressed in several structures, including brain; cardiovascular system; extraembryonic component; genitourinary system; and respiratory system. Used to study COVID-19 and congestive heart failure. Human ortholog(s) of this gene implicated in several diseases, including avian influenza; cerebral malaria; hypertension (multiple); respiratory syncytial virus infectious disease; and severe acute respiratory syndrome. Orthologous to human ACE2 (angiotensin converting enzyme 2).

Rat Ace2 Target Information

Target Symbol: Ace2, angiotensin I converting enzyme 2
Alias:
- ACE-related carboxypeptidase
- angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
- angiotensin-converting enzyme 2
- anigotensin-converting enzyme-related carboxypeptidase
- renal angiotensin-converting enzyme 2
NCBI_Gene: 302668

Rat Ace2 Predicted Functions

Enables carboxypeptidase activity. Involved in maternal process involved in female pregnancy; negative regulation of ERK1 and ERK2 cascade; and negative regulation of smooth muscle cell proliferation. Located in extracellular space and membrane. Used to study glaucoma; myocardial infarction; and thrombosis. Biomarker of hypertension; lung disease; middle cerebral artery infarction; myocardial infarction; and primary biliary cholangitis. Human ortholog(s) of this gene implicated in several diseases, including avian influenza; cerebral malaria; hypertension (multiple); respiratory syncytial virus infectious disease; and severe acute respiratory syndrome. Orthologous to human ACE2 (angiotensin converting enzyme 2).

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