Glucagon ELISA Kit

$245.00 – $350.00

Related Target	GCG
Species	human,mouse,rat
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	8.42 pg/mL
Array Range	78.13 pg/mL – 5000 pg/mL
Assay Time	3.5 h
Recovery	80% – 127%
Average Recovery	102%

ELISA Kit Detail Information

Related Target	GCG
Species	human,mouse,rat
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	8.42 pg/mL
Array Range	78.13 pg/mL – 5000 pg/mL
Assay Time	3.5 h
Recovery	80% – 127%
Average Recovery	102%
Intra Precision	1.5% – 3.7%
Inter Precision	4.4% – 6.2%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Glucagon monoclonal antibody Glucagon freeze-dried standard Glucagon detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-Glucagon antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the Glucagon present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Glucagon in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

GCG

GCG Target Infomation Overview

Target Symbol: GCG, glucagon
Gene Groups: Receptor ligands
Alias: GLP1; GLP2; GRPP; GLP-1

GCG, glucagon Target Infomation by Species

Human
Mouse
Rat

Human GCG Target Information

Target Symbol: GCG, glucagon
Alias:
- glicentin-related polypeptide
- GLP-1
- GLP1
- GLP2
- glucagon-like peptide 1
- glucagon-like peptide 2
- GRPP
- preproglucagon
- pro-glucagon
NCBI_Gene: 2641
UniProtKB: P01275

Human GCG Predicted Functions

Enables gastric inhibitory polypeptide receptor binding activity and identical protein binding activity. Predicted to be involved in several processes, including positive regulation of bicellular tight junction assembly; positive regulation of insulin secretion involved in cellular response to glucose stimulus; and protein kinase A signaling. Predicted to act upstream of or within negative regulation of execution phase of apoptosis; positive regulation of gluconeogenesis; and positive regulation of histone H3-K4 methylation. Predicted to be located in cytoplasm. Predicted to be active in extracellular space. Implicated in Alzheimer’s disease and prediabetes syndrome.

Mouse Gcg Target Information

Target Symbol: Gcg, glucagon
Alias:
- GLP-1
- Glu
- glucagon-like peptide I
- PPG
NCBI_Gene: 14526
- UniprotKB: P55095
- UniprotKB: A2AS86

Mouse Gcg Predicted Functions

Predicted to enable identical protein binding activity and signaling receptor binding activity. Involved in several processes, including positive regulation of protein phosphorylation; positive regulation of transport; and regulation of intracellular signal transduction. Acts upstream of or within several processes, including positive regulation of gluconeogenesis; positive regulation of histone H3-K4 methylation; and regulation of insulin secretion. Located in cytoplasm and extracellular space. Is expressed in several structures, including brain; gut; and urogenital ridge. Human ortholog(s) of this gene implicated in Alzheimer’s disease and prediabetes syndrome. Orthologous to human GCG (glucagon).

Rat Gcg Target Information

Target Symbol: Gcg, glucagon
Alias:
- GLP-1
- Glp1
- glucagon-like peptide-1
- pro-glucagon
NCBI_Gene: 24952
- UniprotKB: G3V6P5
- UniprotKB: P06883

Rat Gcg Predicted Functions

Enables glucagon receptor binding activity and hormone activity. Involved in adenylate cyclase-modulating G protein-coupled receptor signaling pathway; negative regulation of appetite; and response to L-arginine. Located in extracellular space. Human ortholog(s) of this gene implicated in Alzheimer’s disease and prediabetes syndrome. Orthologous to human GCG (glucagon).

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