Name: Human Apolipoprotein D/Apo D ELISA Kit
SKU: EK1223
Availability: InStock

EK1223

Human Apolipoprotein D/Apo D ELISA Kit

$245.00 – $350.00

Related Target	APOD
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	90 μL 1×Assay Buffer and 10 μL prediluted sample
Sensitivity	25.67 pg/mL
Array Range	0.63 ng/mL – 40 ng/mL
Assay Time	3.5 h
Recovery	85% – 103%
Average Recovery	91%

ELISA Kit Detail Information

Related Target	APOD
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	90 μL 1×Assay Buffer and 10 μL prediluted sample
Sensitivity	25.67 pg/mL
Array Range	0.63 ng/mL – 40 ng/mL
Assay Time	3.5 h
Recovery	85% – 103%
Average Recovery	91%
Intra Precision	2.0% – 8.1%
Inter Precision	1.9% - 2.8%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Apolipoprotein D/Apo D monoclonal antibody Human Apolipoprotein D/Apo D freeze-dried standard Apolipoprotein D/Apo D detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Apo D antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Apo D present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Apo D in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

APOD

No description available.

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