Name: Human Apolipoprotein AII/Apo A2 ELISA Kit
SKU: EK1258
Availability: InStock

EK1258

Human Apolipoprotein AII/Apo A2 ELISA Kit

$245.00 – $350.00

Related Target	APOA2
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	90 μL 1×Assay Buffer and 10 μL prediluted sample
Sensitivity	0.53 pg/mL
Array Range	7.81 pg/mL – 500 pg/mL
Assay Time	3.5 h
Recovery	91% – 123%
Average Recovery	106%

ELISA Kit Detail Information

Related Target	APOA2
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	90 μL 1×Assay Buffer and 10 μL prediluted sample
Sensitivity	0.53 pg/mL
Array Range	7.81 pg/mL – 500 pg/mL
Assay Time	3.5 h
Recovery	91% – 123%
Average Recovery	106%
Intra Precision	3.8% – 4.5%
Inter Precision	4.3% – 9.4%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Apolipoprotein AII/Apo A2 monoclonal antibody Human Apolipoprotein AII/Apo A2 freeze-dried standard Apolipoprotein AII/Apo A2 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Apo A2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Apo A2 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Apo A2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

APOA2

APOA2 Target Infomation Overview

Target Symbol: APOA2, apolipoprotein A2
Gene Groups: Apolipoproteins
Alias Names: apolipoprotein A-II

APOA2, apolipoprotein A2 Target Infomation by Species

Human
Mouse
Rat

Human APOA2 Target Information

Target Symbol: APOA2, apolipoprotein A2
Alias:
- Apo-AII
- ApoA-II
- apoAII
- apolipoprotein A-II
NCBI_Gene: 336
UniProtKB: P02652

Human APOA2 Predicted Functions

Enables several functions, including cholesterol binding activity; protein dimerization activity; and signaling receptor binding activity. Contributes to cholesterol transfer activity. Involved in several processes, including lipid transport; plasma lipoprotein particle organization; and regulation of lipid metabolic process. Located in blood microparticle and extracellular exosome. Part of chylomicron; spherical high-density lipoprotein particle; and very-low-density lipoprotein particle. Implicated in familial hypercholesterolemia. Biomarker of coronary artery disease; familial combined hyperlipidemia; obesity; type 1 diabetes mellitus; and type 2 diabetes mellitus.

Mouse Apoa2 Target Information

Target Symbol: Apoa2, apolipoprotein A-II
Alias:
- Alp-2
- Apoa-2
- ApoA-II
- Hdl-1
- high density lipoprotein 1
NCBI_Gene: 11807
- UniprotKB: P09813
- UniprotKB: Q6LD55

Mouse Apoa2 Predicted Functions

Enables high-density lipoprotein particle binding activity. Acts upstream of or within several processes, including cholesterol homeostasis; negative regulation of lipase activity; and regulation of intestinal cholesterol absorption. Located in extracellular space. Is expressed in alimentary system; embryo; liver; liver lobe; and lung. Human ortholog(s) of this gene implicated in familial hypercholesterolemia. Orthologous to human APOA2 (apolipoprotein A2).

Rat Apoa2 Target Information

Target Symbol: Apoa2, apolipoprotein A2
Alias:
- Apo-AII
- ApoA-II
- APOAII
- apolipoprotein A-II
NCBI_Gene: 25649
UniProtKB: P04638

Rat Apoa2 Predicted Functions

Enables cholesterol transfer activity. Involved in several processes, including animal organ regeneration; response to estrogen; and response to glucocorticoid. Predicted to be located in extracellular space. Predicted to be part of chylomicron; spherical high-density lipoprotein particle; and very-low-density lipoprotein particle. Biomarker of hyperthyroidism. Human ortholog(s) of this gene implicated in familial hypercholesterolemia. Orthologous to human APOA2 (apolipoprotein A2).

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