Human ANGPTL4 ELISA Kit

$245.00 – $350.00

Related Target	ANGPTL4
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	43.6 pg/mL
Array Range	0.31 pg/mL – 20 pg/mL
Assay Time	3.5 h
Recovery	95% – 119%
Average Recovery	109%

ELISA Kit Detail Information

Related Target	ANGPTL4
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	43.6 pg/mL
Array Range	0.31 pg/mL – 20 pg/mL
Assay Time	3.5 h
Recovery	95% – 119%
Average Recovery	109%
Intra Precision	4.8% – 4.9%
Inter Precision	4.7% – 5.0%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-ANGPTL4 monoclonal antibody Human ANGPTL4 freeze-dried standard ANGPTL4 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human ANGPTL4 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, ANGPTL4 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of ANGPTL4 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

ANGPTL4

ANGPTL4 Target Infomation Overview

Target Symbol: ANGPTL4, angiopoietin like 4
Gene Groups: Fibrinogen C domain containing; Angiopoietin like family
Alias: pp1158; PGAR; ARP4; HFARP; FIAF; NL2
Alias Names: fasting-induced adipose factor; hepatic angiopoietin-related protein; PPARG angiopoietin related protein; hepatic fibrinogen/angiopoietin-related protein; peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin-related protein; angiopoietin-related protein 4; angiopoietin-like 4

ANGPTL4, angiopoietin like 4 Target Infomation by Species

Human
Mouse
Rat

Human ANGPTL4 Target Information

Target Symbol: ANGPTL4, angiopoietin like 4
Alias:
- angiopoietin-like 4
- angiopoietin-like protein 4
- angiopoietin-related protein 4
- ANGPTL2
- ARP4
- fasting-induced adipose factor
- FIAF
- HARP
- hepatic angiopoietin-related protein
- hepatic fibrinogen/angiopoietin-related protein
- HFARP
- NL2
- peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin-related protein
- PGAR
- pp1158
- PPARG angiopoietin related protein
- TGQTL
- UNQ171
NCBI_Gene: 51129
UniProtKB: Q9BY76

Human ANGPTL4 Predicted Functions

Enables identical protein binding activity. Involved in negative regulation of apoptotic process; negative regulation of lipoprotein lipase activity; and protein unfolding. Located in extracellular space.

Mouse Angptl4 Target Information

Target Symbol: Angptl4, angiopoietin-like 4
Alias:
- BK89
- FIAF
- HFARP
- NG27
NCBI_Gene: 57875

Mouse Angptl4 Predicted Functions

Enables enzyme inhibitor activity. Involved in negative regulation of apoptotic process; negative regulation of lipoprotein lipase activity; and triglyceride homeostasis. Acts upstream of or within negative regulation of endothelial cell apoptotic process. Located in extracellular space. Is expressed in several structures, including brain; genitourinary system; hemolymphoid system gland; integumental system; and musculature. Orthologous to human ANGPTL4 (angiopoietin like 4).

Rat Angptl4 Target Information

Target Symbol: Angptl4, angiopoietin-like 4
Alias:
- angiopoietin-like protein 4
- angiopoietin-related protein 4
- hepatic fibrinogen/angiopoietin-related protein
- HFARP
- LOC362850
NCBI_Gene: 362850
UniProtKB: Q6TMA8

Rat Angptl4 Predicted Functions

Enables identical protein binding activity. Involved in response to hypoxia. Predicted to be located in extracellular region. Predicted to be active in collagen-containing extracellular matrix and extracellular space. Orthologous to human ANGPTL4 (angiopoietin like 4).

Citations for Human ANGPTL4 ELISA Kit

There are no citations for this product yet.

If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.

Citations data is updating...

Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.

ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol

Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.

ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects

Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.