Name: Human Arginase 1/ARG1 ELISA Kit
SKU: EK1316
Availability: InStock

EK1316

Human Arginase 1/ARG1 ELISA Kit

$245.00 – $350.00

Related Target	ARG1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	26.80 pg/mL
Array Range	0.78 ng/mL – 50 ng/mL
Assay Time	3.5 h
Recovery	75% – 124%
Average Recovery	102%

ELISA Kit Detail Information

Related Target	ARG1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	26.80 pg/mL
Array Range	0.78 ng/mL – 50 ng/mL
Assay Time	3.5 h
Recovery	75% – 124%
Average Recovery	102%
Intra Precision	3.4% – 4.6%
Inter Precision	2.6% – 4.8%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-ARG1 monoclonal antibody Human ARG1 freeze-dried standard Angiotensinogen/AGT/ SerpinA8 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human ARG1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, ARG1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of ARG1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

ARG1

ARG1 Target Infomation Overview

Target Symbol: ARG1, arginase 1
Alias Names: arginase-1; arginase, liver

ARG1, arginase 1 Target Infomation by Species

Human
Mouse
Rat

Human ARG1 Target Information

Target Symbol: ARG1, arginase 1
Alias:
- arginase-1
- arginase, liver
- liver-type arginase
- type I arginase
NCBI_Gene: 383
UniProtKB: P05089

Human ARG1 Predicted Functions

Predicted to enable arginase activity and manganese ion binding activity. Involved in negative regulation of T cell proliferation; negative regulation of interferon-gamma-mediated signaling pathway; and positive regulation of neutrophil mediated killing of fungus. Located in extracellular space. Implicated in asthma; hepatocellular carcinoma; and hyperargininemia. Biomarker of asthma and colitis.

Mouse Arg1 Target Information

Target Symbol: Arg1, arginase, liver
Alias:
- AI
- AI256583
- Arg-1
- expressed sequence AI256583
- PGIF
NCBI_Gene: 11846
- UniprotKB: Q61176

Mouse Arg1 Predicted Functions

Enables arginase activity. Involved in defense response to protozoan; negative regulation of T-helper 2 cell cytokine production; and negative regulation of activated T cell proliferation. Predicted to be located in several cellular components, including extracellular space; mitochondrial outer membrane; and neuronal cell body. Predicted to be active in cytosol. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; integumental system; and sensory organ. Used to study hyperargininemia. Human ortholog(s) of this gene implicated in asthma; hepatocellular carcinoma; and hyperargininemia. Orthologous to human ARG1 (arginase 1).

Rat Arg1 Target Information

Target Symbol: Arg1, arginase 1
Alias:
- AI type I arginase
- AI, type I arginase
- arginase 1 liver
- Arginase 1, liver
- arginase-1
- arginase, liver
- liver-type arginase
- type I arginase
NCBI_Gene: 29221
UniProtKB: P07824

Rat Arg1 Predicted Functions

Enables arginase activity; identical protein binding activity; and manganese ion binding activity. Involved in several processes, including cellular response to hormone stimulus; gland development; and response to vitamin. Located in several cellular components, including extracellular space; mitochondrial outer membrane; and neuronal cell body. Used to study acute necrotizing pancreatitis and hypertension. Biomarker of several diseases, including acute kidney failure; cholestasis; silicosis; ureteral obstruction; and uveitis. Human ortholog(s) of this gene implicated in asthma; hepatocellular carcinoma; and hyperargininemia. Orthologous to human ARG1 (arginase 1).

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