Name: Human 4-1BB/TNFRSF9/CD137 ELISA Kit
SKU: EK1263
Availability: InStock

EK1263

Human 4-1BB/TNFRSF9/CD137 ELISA Kit

$245.00 – $350.00

Related Target	TNFRSF9
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	1.58 pg/mL
Array Range	4.69 pg/mL – 300 pg/mL
Assay Time	3.5 h
Recovery	85% – 117%
Average Recovery	102%

ELISA Kit Detail Information

Related Target	TNFRSF9
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	1.58 pg/mL
Array Range	4.69 pg/mL – 300 pg/mL
Assay Time	3.5 h
Recovery	85% – 117%
Average Recovery	102%
Intra Precision	4.7% – 5.0%
Inter Precision	3.2% – 6.6%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-4-1BB/TNFRSF9/CD137 monoclonal antibody Human 4-1BB/TNFRSF9/CD137 freeze-dried standard 4-1BB/TNFRSF9/CD137 detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human 4-1BB antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, 4-1BB present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of 4-1BB in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

TNFRSF9

TNFRSF9 Target Infomation Overview

Target Symbol: TNFRSF9, TNF receptor superfamily member 9
Gene Groups: CD molecules; Tumor necrosis factor receptor superfamily
Alias: CD137; 4-1BB
Previous Names: ILA
Alias Names: tumor necrosis factor receptor superfamily, member 9

TNFRSF9, TNF receptor superfamily member 9 Target Infomation by Species

Human
Mouse
Rat

Human TNFRSF9 Target Information

Target Symbol: TNFRSF9, TNF receptor superfamily member 9
Alias:
- 4-1BB
- 4-1BB ligand receptor
- CD137
- CD137 antigen
- CDw137
- FLJ43501
- homolog of mouse 4-1BB
- ILA
- induced by lymphocyte activation (ILA)
- interleukin-activated receptor, homolog of mouse Ly63
- MGC2172
- receptor protein 4-1BB
- T cell antigen ILA
- T-cell antigen 4-1BB homolog
- T-cell antigen ILA
- tumor necrosis factor receptor superfamily member 9
- tumor necrosis factor receptor superfamily, member 9
NCBI_Gene: 3604
UniProtKB: Q07011

Human TNFRSF9 Predicted Functions

Predicted to enable cytokine binding activity; identical protein binding activity; and signaling receptor activity. Predicted to be involved in regulation of cell population proliferation. Predicted to act upstream of or within negative regulation of interleukin-10 production and negative regulation of interleukin-12 production. Predicted to be located in extracellular space. Predicted to be active in external side of plasma membrane.

Mouse Tnfrsf9 Target Information

Target Symbol: Tnfrsf9, tumor necrosis factor receptor superfamily, member 9
Alias:
- 4-1BB
- A930040I11Rik
- AA408498
- AI325004
- CD antigen 137
- Cd137
- CDw137
- expressed sequence AA408498
- expressed sequence AI325004
- ILA
- Ly63
- lymphocyte antigen 63
- RIKEN cDNA A930040I11 gene
NCBI_Gene: 21942

Mouse Tnfrsf9 Predicted Functions

Enables cytokine binding activity; identical protein binding activity; and signaling receptor activity. Acts upstream of or within negative regulation of interleukin-10 production; negative regulation of interleukin-12 production; and regulation of cell population proliferation. Located in external side of plasma membrane and extracellular space. Is expressed in renal vasculature. Orthologous to human TNFRSF9 (TNF receptor superfamily member 9).

Rat Tnfrsf9 Target Information

Target Symbol: Tnfrsf9, TNF receptor superfamily member 9
Alias:
- 4-1BB
- LOC500590
- MGC114552
- similar to T-cell antigen 4-1BB precursor – mouse
- tumor necrosis factor receptor superfamily member 9
- tumor necrosis factor receptor superfamily, member 9
NCBI_Gene: 500590
UniProtKB: Q4V895

Rat Tnfrsf9 Predicted Functions

Predicted to enable cytokine binding activity; identical protein binding activity; and signaling receptor activity. Predicted to be involved in regulation of cell population proliferation. Predicted to act upstream of or within negative regulation of interleukin-10 production and negative regulation of interleukin-12 production. Predicted to be located in extracellular space and plasma membrane. Predicted to be active in external side of plasma membrane. Orthologous to human TNFRSF9 (TNF receptor superfamily member 9).

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