Name: Cortisol Competitive ELISA Kit
SKU: EK8100
Availability: InStock

EK8100

Cortisol Competitive ELISA Kit

$245.00 – $350.00

Species	human,mouse,rat
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	66.43 pg/mL
Array Range	0.12 ng/mL – 15 ng/mL
Assay Time	3.5 h
Recovery	80% – 130%
Average Recovery	118%

ELISA Kit Detail Information

Species	human,mouse,rat
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	66.43 pg/mL
Array Range	0.12 ng/mL – 15 ng/mL
Assay Time	3.5 h
Recovery	80% – 130%
Average Recovery	118%
Intra Precision	4.3% – 4.6%
Inter Precision	6.4% – 7.8%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Competitive monoclonal antibody Cortisol competitive freeze-dried standard Competitive detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes a competitive enzyme-linked immunosorbent assay (ELISA) technique. Rabbit anti-mouse antibody is precoated on a high-affinity ELISA plate. Cortisol-specific monoclonal antibodies are added to the wells of the plate and incubated, allowing them to bind to the solid-phase antibodies. After washing, HRP-labeled cortisol and unlabeled cortisol or samples are added, competing for limited binding sites on the monoclonal antibody. After washing to remove unbound substances, a chromogenic substrate is added, and the intensity of the color reaction is inversely proportional to the concentration of cortisol in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). This assay kit has been validated for human, mouse, and rat serum samples, but cortisol from other species is also expected to be detectable.

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