Name: Human Antithrombin-III/Serpin C1 ELISA Kit
SKU: EK1275
Availability: InStock

EK1275

Human Antithrombin-III/Serpin C1 ELISA Kit

$245.00 – $350.00

Related Target	SERPINC1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	6.15 pg/mL
Array Range	0.16 ng/mL – 10 ng/mL
Assay Time	3.5 h
Recovery	88% – 114%
Average Recovery	101%

ELISA Kit Detail Information

Related Target	SERPINC1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	6.15 pg/mL
Array Range	0.16 ng/mL – 10 ng/mL
Assay Time	3.5 h
Recovery	88% – 114%
Average Recovery	101%
Intra Precision	4.4% – 4.9%
Inter Precision	4.4% - 4.9%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Antithrombin-III/Serpin C1 monoclonal antibody Human Antithrombin-III/Serpin C1 freeze-dried standard Antithrombin-III/Serpin C1 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Antithrombin-III antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the Antithrombin-III present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Antithrombin-III in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

SERPINC1

SERPINC1 Target Infomation Overview

Target Symbol: SERPINC1, serpin family C member 1
Gene Groups: Serpin peptidase inhibitors
Alias: ATIII; MGC22579
Previous Names: AT3
Alias Names: antithrombin III; signal peptide antithrombin part 1; coding sequence signal peptide antithrombin part 1; antithrombin (aa 375-432); serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1; serpin peptidase inhibitor, clade C (antithrombin), member 1

SERPINC1, serpin family C member 1 Target Infomation by Species

Human
Mouse
Rat

Human SERPINC1 Target Information

Target Symbol: SERPINC1, serpin family C member 1
Alias:
- antithrombin III
- antithrombin III isoform
- antithrombin-III
- AT3
- AT3D
- ATIII
- ATIII-R2
- ATIII-T1
- ATIII-T2
- MGC22579
- serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1
- serine-cysteine proteinase inhibitor clade C member 1
- serpin C1
- serpin peptidase inhibitor clade C member 1
- serpin peptidase inhibitor, clade C (antithrombin), member 1
- THPH7
NCBI_Gene: 462
UniProtKB: P01008

Human SERPINC1 Predicted Functions

Enables identical protein binding activity and protease binding activity. Predicted to be involved in negative regulation of endopeptidase activity. Located in extracellular space. Part of collagen-containing extracellular matrix. Implicated in antithrombin III deficiency; disseminated intravascular coagulation; intermediate coronary syndrome; thrombosis; and toxic shock syndrome. Biomarker of acute kidney failure; nephrotic syndrome; osteonecrosis; and type 2 diabetes mellitus.

Mouse Serpinc1 Target Information

Target Symbol: Serpinc1, serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1
Alias:
- AI114908
- anti-thrombin 3
- antithrombin
- At-3
- At3
- ATIII
- expressed sequence AI114908
NCBI_Gene: 11905

Mouse Serpinc1 Predicted Functions

Predicted to enable several functions, including heparin binding activity; identical protein binding activity; and serine-type endopeptidase inhibitor activity. Predicted to be involved in negative regulation of endopeptidase activity and response to nutrient. Predicted to act upstream of or within blood coagulation and negative regulation of peptidase activity. Located in collagen-containing extracellular matrix. Is expressed in embryo; liver; neural tube; and notochord. Used to study antithrombin III deficiency. Human ortholog(s) of this gene implicated in antithrombin III deficiency; disseminated intravascular coagulation; intermediate coronary syndrome; thrombosis; and toxic shock syndrome. Orthologous to human SERPINC1 (serpin family C member 1).

Rat Serpinc1 Target Information

Target Symbol: Serpinc1, serpin family C member 1
Alias:
- antithrombin III
- antithrombin-III
- LOC304917
- serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1
- serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1
- serpin peptidase inhibitor, clade C (antithrombin), member 1
NCBI_Gene: 304917
- UniprotKB: A0A096MIW4
- UniprotKB: Q5M7T5

Rat Serpinc1 Predicted Functions

Enables heparin binding activity and serine-type endopeptidase inhibitor activity. Involved in acute inflammatory response to antigenic stimulus; lactation; and response to nutrient. Located in extracellular space. Used to study thrombosis. Biomarker of familial hyperlipidemia; nephrotic syndrome; and ovarian cancer. Human ortholog(s) of this gene implicated in antithrombin III deficiency; disseminated intravascular coagulation; intermediate coronary syndrome; thrombosis; and toxic shock syndrome. Orthologous to human SERPINC1 (serpin family C member 1).

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