Name: Human Apolipoprotein M/Apo M ELISA Kit
SKU: EK1259
Availability: InStock

EK1259

Human Apolipoprotein M/Apo M ELISA Kit

$245.00 – $350.00

Related Target	APOM
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	2.44 pg/mL
Array Range	0.16 ng/mL – 10 ng/mL
Assay Time	3.5 h
Recovery	90% – 109%
Average Recovery	100%

ELISA Kit Detail Information

Related Target	APOM
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	2.44 pg/mL
Array Range	0.16 ng/mL – 10 ng/mL
Assay Time	3.5 h
Recovery	90% – 109%
Average Recovery	100%
Intra Precision	2.1% – 3.9%
Inter Precision	3.4% - 4.3%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Apolipoprotein M/Apo M monoclonal antibody Human Apolipoprotein M/Apo M freeze-dried standard Human Apolipoprotein M/Apo M detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Apo M antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Apo M present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Apo M in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

APOM

APOM Target Infomation Overview

Target Symbol: APOM, apolipoprotein M
Gene Groups: Apolipoproteins; Lipocalins
Alias: G3a; NG20

APOM, apolipoprotein M Target Infomation by Species

Human
Mouse
Rat

Human APOM Target Information

Target Symbol: APOM, apolipoprotein M
Alias:
- alternative name: G3a, NG20
- apo-M
- G3a
- HSPC336
- MGC22400
- NG20
- NG20-like protein
NCBI_Gene: 55937
UniProtKB: O95445

Human APOM Predicted Functions

Enables antioxidant activity; lipid transporter activity; and phospholipid binding activity. Involved in cholesterol efflux; high-density lipoprotein particle remodeling; and negative regulation of plasma lipoprotein oxidation. Part of high-density lipoprotein particle; low-density lipoprotein particle; and very-low-density lipoprotein particle. Implicated in coronary artery disease; type 1 diabetes mellitus; and type 2 diabetes mellitus.

Mouse Apom Target Information

Target Symbol: Apom, apolipoprotein M
Alias:
- 1190010O19Rik
- G3a
- NG20
- RIKEN cDNA 1190010O19 gene
NCBI_Gene: 55938
- UniprotKB: Q9Z1R3
- UniprotKB: Q8VC79

Mouse Apom Predicted Functions

Enables antioxidant activity. Involved in several processes, including cholesterol efflux; high-density lipoprotein particle clearance; and plasma lipoprotein particle organization. Acts upstream of or within lipoprotein metabolic process and reverse cholesterol transport. Located in extracellular region. Part of discoidal high-density lipoprotein particle. Is expressed in several structures, including gut; liver; male reproductive gland or organ; and urinary system. Human ortholog(s) of this gene implicated in coronary artery disease; type 1 diabetes mellitus; and type 2 diabetes mellitus. Orthologous to human APOM (apolipoprotein M).

Rat Apom Target Information

Target Symbol: Apom, apolipoprotein M
Alias:
- apo-M
- protein Px
NCBI_Gene: 55939
UniProtKB: P14630

Rat Apom Predicted Functions

Predicted to enable antioxidant activity; lipid transporter activity; and phospholipid binding activity. Involved in response to glucose. Located in extracellular space. Human ortholog(s) of this gene implicated in coronary artery disease; type 1 diabetes mellitus; and type 2 diabetes mellitus. Orthologous to human APOM (apolipoprotein M).

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