EasyGo!™ Mouse IL-18 One-Step ELISA Kit

EasyGo! One-Step ELISA Kits

$400.00 – $500.00

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Datasheet

SDS File

TARGETS	IL18
SPECIES	Mouse
Sample Type	Serum, plasma, DMEM cell culture supernatant
Sample Volume	Serum or plasma: 20μL; DMEM cell culture supernatant: 50μL
Sensitivity	0.35 pg/mL
Range	3.125 pg/mL – 200 pg/mL
Assay Time	1.5 h
Recovery	88% – 114%
Average Recovery	0.99

Sample Type	Serum, plasma, DMEM cell culture supernatant
Sample Volume	Serum or plasma: 20μL; DMEM cell culture supernatant: 50μL
Sensitivity	0.35 pg/mL
Range	3.125 pg/mL – 200 pg/mL
Assay Time	1.5 h
Recovery	88% – 114%
Average Recovery	0.99
Intra Precision	3.4% – 4.6%
Inter-Precision	3.1% – 5.4%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL18 monoclonal antibody Human IL18 freeze-dried standard IL18 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilizedantibody. The color development is stopped and the intensity of the color is measured bymicroplate reader.

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Targets

IL18

IL18 Target Infomation Overview

Target Symbol: IL18, interleukin 18
Gene Groups: Interleukins
Alias: IGIF; IL1F4; IL-1g; IL-18
Alias Names: interferon-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor)

IL18, interleukin 18 Target Infomation by Species

HumanMouseRat

Human IL18 Target Information

Target Symbol: IL18, interleukin 18
Alias:
- iboctadekin
- IFN-gamma-inducing factor
- IGIF
- IL-1 gamma
- IL-18
- IL-1g
- IL1F4
- interferon gamma-inducing factor
- interleukin 18 (interferon-gamma-inducing factor)
- interleukin-1 gamma
- interleukin-18
- MGC12320
NCBI_Gene: 3606
UniProtKB: Q14116

Human IL18 Predicted Functions

Enables cytokine activity and interleukin-18 receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Located in extracellular space. Implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Biomarker of several diseases, including autoimmune disease (multiple); cholestasis (multiple); liver disease (multiple); lung disease (multiple); and myositis (multiple).

Mouse Il18 Target Information

Target Symbol: Il18, interleukin 18
Alias:
- Igif
- Il-18
- interferon gamma inducing factor
NCBI_Gene: 16173

Mouse Il18 Predicted Functions

Enables cytokine activity. Involved in lipid homeostasis; positive regulation of cell differentiation; and positive regulation of cold-induced thermogenesis. Acts upstream of or within several processes, including interleukin-18-mediated signaling pathway; positive regulation of NIK/NF-kappaB signaling; and positive regulation of interferon-gamma production. Located in extracellular space. Is expressed in several structures, including back skin; dorsal aorta; gut; lymphatic system; and reproductive system. Used to study atopic dermatitis. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Orthologous to human IL18 (interleukin 18).

Rat Il18 Target Information

Target Symbol: Il18, interleukin 18
Alias:
- IFN-gamma-inducing factor
- IL-1 gamma
- IL-18
- interferon gamma-inducing factor
- interferon-gamma-inducing factor
- interleukin-1 gamma
- interleukin-18
NCBI_Gene: 29197
- UniprotKB: P97636
- UniprotKB: Z4YNI9

Rat Il18 Predicted Functions

Predicted to enable cytokine activity and interleukin-18 receptor binding activity. Involved in several processes, including cellular response to hormone stimulus; positive regulation of cell population proliferation; and positive regulation of cytokine production. Located in apical plasma membrane; cytoplasm; and extracellular space. Used to study brain edema; fatty liver disease; heart disease; status epilepticus; and vasculitis. Biomarker of several diseases, including cardiac arrest; ischemia (multiple); lung disease (multiple); perinatal necrotizing enterocolitis; and urinary tract infection. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Orthologous to human IL18 (interleukin 18).