Mouse IL-18 ELISA Kit
$245.00 – $350.00
Related Target | |
---|---|
Species | mouse |
Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
Sample Volume | Serum, plasma: 20 μL, cell culture supernatant: 100 μL |
Sensitivity | 0.93 pg/mL |
Array Range | 62.5 pg/mL – 4000 pg/mL |
Assay Time | 3.5 h |
Recovery | 80% – 119% |
Average Recovery | 98% |
ELISA Kit Detail Information
Related Target | |
---|---|
Species | mouse |
Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
Sample Volume | Serum, plasma: 20 μL, cell culture supernatant: 100 μL |
Sensitivity | 0.93 pg/mL |
Array Range | 62.5 pg/mL – 4000 pg/mL |
Assay Time | 3.5 h |
Recovery | 80% – 119% |
Average Recovery | 98% |
Intra Precision | 2.7% – 4.0% |
Inter Precision | 2.4% – 3.7% |
Plate | Detachable 96-well plate |
Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
Delivery | 4℃ blue ice transportation |
Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Mouse IL-18 monoclonal antibody Mouse IL-18 freeze-dried standard IL-18 detect Antibody HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-18 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, IL-18 present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-18 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
IL18
IL18 Target Infomation Overview
- Target Symbol: IL18, interleukin 18
- Gene Groups: Interleukins
- Alias: IGIF; IL1F4; IL-1g; IL-18
- Alias Names: interferon-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor)
IL18, interleukin 18 Target Infomation by Species
- Human
- Mouse
- Rat
Human IL18 Target Information
- Target Symbol: IL18, interleukin 18
- Alias:
- iboctadekin
- IFN-gamma-inducing factor
- IGIF
- IL-1 gamma
- IL-18
- IL-1g
- IL1F4
- interferon gamma-inducing factor
- interleukin 18 (interferon-gamma-inducing factor)
- interleukin-1 gamma
- interleukin-18
- MGC12320
- NCBI_Gene: 3606
- UniProtKB: Q14116
Human IL18 Predicted Functions
Enables cytokine activity and interleukin-18 receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Located in extracellular space. Implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Biomarker of several diseases, including autoimmune disease (multiple); cholestasis (multiple); liver disease (multiple); lung disease (multiple); and myositis (multiple).
Mouse Il18 Target Information
- Target Symbol: Il18, interleukin 18
- Alias:
- Igif
- Il-18
- interferon gamma inducing factor
- NCBI_Gene: 16173
Mouse Il18 Predicted Functions
Enables cytokine activity. Involved in lipid homeostasis; positive regulation of cell differentiation; and positive regulation of cold-induced thermogenesis. Acts upstream of or within several processes, including interleukin-18-mediated signaling pathway; positive regulation of NIK/NF-kappaB signaling; and positive regulation of interferon-gamma production. Located in extracellular space. Is expressed in several structures, including back skin; dorsal aorta; gut; lymphatic system; and reproductive system. Used to study atopic dermatitis. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Orthologous to human IL18 (interleukin 18).
Rat Il18 Target Information
- Target Symbol: Il18, interleukin 18
- Alias:
- IFN-gamma-inducing factor
- IL-1 gamma
- IL-18
- interferon gamma-inducing factor
- interferon-gamma-inducing factor
- interleukin-1 gamma
- interleukin-18
- NCBI_Gene: 29197
Rat Il18 Predicted Functions
Predicted to enable cytokine activity and interleukin-18 receptor binding activity. Involved in several processes, including cellular response to hormone stimulus; positive regulation of cell population proliferation; and positive regulation of cytokine production. Located in apical plasma membrane; cytoplasm; and extracellular space. Used to study brain edema; fatty liver disease; heart disease; status epilepticus; and vasculitis. Biomarker of several diseases, including cardiac arrest; ischemia (multiple); lung disease (multiple); perinatal necrotizing enterocolitis; and urinary tract infection. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Orthologous to human IL18 (interleukin 18).
Citations for Mouse IL-18 ELISA Kit
There are no citations for this product yet.
If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.
Citations data is updating...
Reviews
There are no reviews yet.