Powered by BiozMouse IL-18 ELISA Kit
$299.00 – $419.00
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | Serum, plasma: 20 μL, cell culture supernatant: 100 μL |
| Sensitivity | 0.93 pg/mL |
| Array Range | 62.5 pg/mL – 4000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 80% – 119% |
| Average Recovery | 98% |
| Intra Precision | 2.7% – 4.0% |
| Inter Precision | 2.4% – 3.7% |
| Plate | Detachable 96-well plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Mouse IL-18 monoclonal antibody Mouse IL-18 freeze-dried standard IL-18 detect Antibody HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-18 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, IL-18 present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-18 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
IL18
IL18 Target Infomation Overview
- Target Symbol: IL18, interleukin 18
- Gene Groups: Interleukins
- Alias: IGIF; IL1F4; IL-1g; IL-18
- Alias Names: interferon-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor)
IL18, interleukin 18 Target Infomation by Species
- Human
- Mouse
- Rat
Human IL18 Target Information
- Target Symbol: IL18, interleukin 18
- Alias:
- iboctadekin
- IFN-gamma-inducing factor
- IGIF
- IL-1 gamma
- IL-18
- IL-1g
- IL1F4
- interferon gamma-inducing factor
- interleukin 18 (interferon-gamma-inducing factor)
- interleukin-1 gamma
- interleukin-18
- MGC12320
- NCBI_Gene: 3606
- UniProtKB: Q14116
Human IL18 Predicted Functions
Enables cytokine activity and interleukin-18 receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Located in extracellular space. Implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Biomarker of several diseases, including autoimmune disease (multiple); cholestasis (multiple); liver disease (multiple); lung disease (multiple); and myositis (multiple).
Mouse Il18 Target Information
- Target Symbol: Il18, interleukin 18
- Alias:
- Igif
- Il-18
- interferon gamma inducing factor
- NCBI_Gene: 16173
Mouse Il18 Predicted Functions
Enables cytokine activity. Involved in lipid homeostasis; positive regulation of cell differentiation; and positive regulation of cold-induced thermogenesis. Acts upstream of or within several processes, including interleukin-18-mediated signaling pathway; positive regulation of NIK/NF-kappaB signaling; and positive regulation of interferon-gamma production. Located in extracellular space. Is expressed in several structures, including back skin; dorsal aorta; gut; lymphatic system; and reproductive system. Used to study atopic dermatitis. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Orthologous to human IL18 (interleukin 18).
Rat Il18 Target Information
- Target Symbol: Il18, interleukin 18
- Alias:
- IFN-gamma-inducing factor
- IL-1 gamma
- IL-18
- interferon gamma-inducing factor
- interferon-gamma-inducing factor
- interleukin-1 gamma
- interleukin-18
- NCBI_Gene: 29197
Rat Il18 Predicted Functions
Predicted to enable cytokine activity and interleukin-18 receptor binding activity. Involved in several processes, including cellular response to hormone stimulus; positive regulation of cell population proliferation; and positive regulation of cytokine production. Located in apical plasma membrane; cytoplasm; and extracellular space. Used to study brain edema; fatty liver disease; heart disease; status epilepticus; and vasculitis. Biomarker of several diseases, including cardiac arrest; ischemia (multiple); lung disease (multiple); perinatal necrotizing enterocolitis; and urinary tract infection. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Orthologous to human IL18 (interleukin 18).
Products with the Same Target - IL18
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
Related products
-
-
- EK195
- ELISA Kit
Human Adiponectin ELISA Kit
- $299.00 – $419.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1299
- ELISA Kit
hCG ELISA Kit
- $299.00 – $419.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1109
- ELISA Kit
Human sTNF RI/TNFRSF1A ELISA Kit
- $299.00 – $419.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1292
- ELISA Kit
Human ANGPTL4 ELISA Kit
- $299.00 – $419.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1275
- ELISA Kit
Human Antithrombin-III/Serpin C1 ELISA Kit
- $299.00 – $419.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1188
- ELISA Kit
Human B4GALT1/GGTB2 ELISA Kit
- $299.00 – $419.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
Reviews
There are no reviews yet.