EK218EGB

EasyGo!™ Mouse IL-18 One-Step ELISA Kit

$349.00$499.00

Related Target
Speciesmouse
Sample Typecell culture supernatant
Sample Volumecell culture supernatant: 100 μL
Sensitivity3.47 pg/mL
Array Range31.25 - 2000 pg/ml
Assay Time1.5h
Recovery76% - 118%
Average Recovery97%
SKU: EK218EGB Category:

ELISA Kit Detail Information

Related Target
Speciesmouse
Sample Typecell culture supernatant
Sample Volumecell culture supernatant: 100 μL
Sensitivity3.47 pg/mL
Array Range31.25 - 2000 pg/ml
Assay Time1.5h
Recovery76% - 118%
Average Recovery97%
Intra Precision2.7% - 2.8%
Inter Precision3.5% - 4.6%
PlateDetachable 96-well Plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ Blue Ice Transport
Components1× Washing Buffer
1× Assay Buffer
Stop Solution
Substrate TMB
Standard
Microplate Wells
Assay PrincipleThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader.

Related Targets

IL18

IL18 Target Infomation Overview

  • Target Symbol: IL18, interleukin 18
  • Gene Groups: Interleukins
  • Alias: IGIF; IL1F4; IL-1g; IL-18
  • Alias Names: interferon-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor)

IL18, interleukin 18 Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human IL18 Target Information

  • Target Symbol: IL18, interleukin 18
  • Alias:
    • iboctadekin
    • IFN-gamma-inducing factor
    • IGIF
    • IL-1 gamma
    • IL-18
    • IL-1g
    • IL1F4
    • interferon gamma-inducing factor
    • interleukin 18 (interferon-gamma-inducing factor)
    • interleukin-1 gamma
    • interleukin-18
    • MGC12320
  • NCBI_Gene: 3606
  • UniProtKB: Q14116

Human IL18 Predicted Functions

Enables cytokine activity and interleukin-18 receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Located in extracellular space. Implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Biomarker of several diseases, including autoimmune disease (multiple); cholestasis (multiple); liver disease (multiple); lung disease (multiple); and myositis (multiple).

Mouse Il18 Target Information

Mouse Il18 Predicted Functions

Enables cytokine activity. Involved in lipid homeostasis; positive regulation of cell differentiation; and positive regulation of cold-induced thermogenesis. Acts upstream of or within several processes, including interleukin-18-mediated signaling pathway; positive regulation of NIK/NF-kappaB signaling; and positive regulation of interferon-gamma production. Located in extracellular space. Is expressed in several structures, including back skin; dorsal aorta; gut; lymphatic system; and reproductive system. Used to study atopic dermatitis. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Orthologous to human IL18 (interleukin 18).

Rat Il18 Target Information

Rat Il18 Predicted Functions

Predicted to enable cytokine activity and interleukin-18 receptor binding activity. Involved in several processes, including cellular response to hormone stimulus; positive regulation of cell population proliferation; and positive regulation of cytokine production. Located in apical plasma membrane; cytoplasm; and extracellular space. Used to study brain edema; fatty liver disease; heart disease; status epilepticus; and vasculitis. Biomarker of several diseases, including cardiac arrest; ischemia (multiple); lung disease (multiple); perinatal necrotizing enterocolitis; and urinary tract infection. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Orthologous to human IL18 (interleukin 18).

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