EasyGo!™ Mouse IL-4 One-Step ELISA Kit
$294.00 – $420.00
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma |
| Sample Volume | Serum, plasma: 5 μL |
| Sensitivity | 5.75 pg/mL |
| Array Range | 31.25 pg/mL - 2000 pg/mL |
| Assay Time | 1.5h |
| Recovery | 76% - 123% |
| Average Recovery | 96% |
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma |
| Sample Volume | Serum, plasma: 5 μL |
| Sensitivity | 5.75 pg/mL |
| Array Range | 31.25 pg/mL - 2000 pg/mL |
| Assay Time | 1.5h |
| Recovery | 76% - 123% |
| Average Recovery | 96% |
| Intra Precision | 3.5% - 6.6% |
| Inter Precision | 6.8% - 8.3% |
| Plate | Detachable 96-well Plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ Blue Ice Transport |
| Components | 1× Washing Buffer 1× Assay Buffer Stop Solution Substrate TMB Standard Microplate Wells |
| Assay Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-4 has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for IL-4 (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-4 present is bound by the immobilized antibody and detect antibody, of which the latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-4 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader. |
Related Targets
IL4
IL4 Target Infomation Overview
- Target Symbol: IL4, interleukin 4
- Gene Groups: Interleukins
- Alias: BSF1; IL-4; BCGF1; BCGF-1; MGC79402
- Alias Names: B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; B cell growth factor 1
IL4, interleukin 4 Target Infomation by Species
- Human
- Mouse
- Rat
Human IL4 Target Information
- Target Symbol: IL4, interleukin 4
- Alias:
- B cell growth factor 1
- B_cell stimulatory factor 1
- B-cell stimulatory factor 1
- BCGF-1
- BCGF1
- binetrakin
- BSF-1
- BSF1
- IL-4
- interleukin 4 variant 2
- interleukin-4
- lymphocyte stimulatory factor 1
- MGC79402
- pitrakinra
- NCBI_Gene: 3565
- UniProtKB: P05112
Human IL4 Predicted Functions
Predicted to enable cytokine activity. Involved in several processes, including negative regulation of cellular response to transforming growth factor beta stimulus; negative regulation of complement-dependent cytotoxicity; and positive regulation of macromolecule metabolic process. Acts upstream of or within positive regulation of cell migration and positive regulation of cell population proliferation. Predicted to be located in external side of plasma membrane. Predicted to be active in extracellular space. Implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); hepatitis B; hepatitis C; and pancreatic cancer (multiple). Biomarker of several diseases, including allergic rhinitis; alopecia universalis; anogenital venereal wart; autoimmune disease (multiple); and liver disease (multiple).
Mouse Il4 Target Information
- Target Symbol: Il4, interleukin 4
- Alias:
- Il-4
- NCBI_Gene: 16189
Mouse Il4 Predicted Functions
Enables cytokine activity. Involved in several processes, including innate immune response in mucosa; negative regulation of white fat cell proliferation; and regulation of gene expression. Acts upstream of or within several processes, including T-helper cell differentiation; positive regulation of macromolecule metabolic process; and regulation of leukocyte activation. Located in external side of plasma membrane and extracellular space. Is expressed in several structures, including brain; colon; hemolymphoid system; liver; and placenta. Used to study Sjogren’s syndrome; atopic dermatitis; and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); hepatitis B; hepatitis C; and pancreatic cancer (multiple). Orthologous to human IL4 (interleukin 4).
Rat Il4 Target Information
- Target Symbol: Il4, interleukin 4
- Alias:
- B-cell growth factor 1
- B-cell IGG differentiation factor
- B-cell stimulatory factor 1
- BSF-1
- IL-4
- Il4e12
- interleukin-4
- lymphocyte stimulatory factor 1
- NCBI_Gene: 287287
Rat Il4 Predicted Functions
Predicted to enable cytokine activity. Involved in several processes, including positive regulation of biosynthetic process; positive regulation of leukocyte migration; and regulation of defense response. Located in extracellular space. Used to study anti-basement membrane glomerulonephritis; autoimmune thyroiditis; and pelvic inflammatory disease. Biomarker of several diseases, including cerebrovascular disease (multiple); interstitial lung disease (multiple); irritant dermatitis; primary biliary cholangitis; and rhinitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); hepatitis B; hepatitis C; and pancreatic cancer (multiple). Orthologous to human IL4 (interleukin 4).
Products with the Same Target - IL4
Citations for EasyGo!™ Mouse IL-4 One-Step ELISA Kit
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Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
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