EK1138EG

EasyGo!™ Human TIMP-1 One-Step ELISA Kit

$349.00$499.00

 DATASHEET
Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100uL(diluent)
Sensitivity1.43pg/ml
Array Range15.63-1000pg/ml
Assay Time90min
Recovery70%-130%
Average Recovery/
SKU: EK1138EG Category:

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100uL(diluent)
Sensitivity1.43pg/ml
Array Range15.63-1000pg/ml
Assay Time90min
Recovery70%-130%
Average Recovery/
Intra Precision< 10%
Inter Precision< 10%
PlateDetachable 96-well Plate
Storage2-8℃
Deliveryblue ice
ComponentsCoated Microplate
standard
Assay Buffer (10×)
TMB
Stop Solution
Washing Buffer (20×)
Assay PrincipleThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader.

Related Targets

TIMP1

TIMP1 Target Infomation Overview

  • Target Symbol: TIMP1, TIMP metallopeptidase inhibitor 1
  • Gene Groups: Tissue inhibitor of metallopeptidases
  • Alias: EPO
  • Previous Names: TIMP; CLGI
  • Alias Names: tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor)

TIMP1, TIMP metallopeptidase inhibitor 1 Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human TIMP1 Target Information

  • Target Symbol: TIMP1, TIMP metallopeptidase inhibitor 1
  • Alias:
    • CLGI
    • collagenase inhibitor
    • EPA
    • EPO
    • erythroid potentiating activity
    • erythroid-potentiating activity
    • fibroblast collagenase inhibitor
    • FLJ90373
    • HCI
    • metalloproteinase inhibitor 1
    • TIMP
    • TIMP-1
    • tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor)
    • tissue inhibitor of metalloproteinases 1
  • NCBI_Gene: 7076
  • UniProtKB: P01033

Human TIMP1 Predicted Functions

Enables cytokine activity; metalloendopeptidase inhibitor activity; and zinc ion binding activity. Involved in several processes, including cellular response to UV-A; negative regulation of proteolysis; and negative regulation of trophoblast cell migration. Located in extracellular space. Biomarker of several diseases, including CAKUT2; Down syndrome; artery disease (multiple); carcinoma (multiple); and diabetes mellitus (multiple).

Mouse Timp1 Target Information

Mouse Timp1 Predicted Functions

Predicted to enable several functions, including cytokine activity; metalloendopeptidase inhibitor activity; and zinc ion binding activity. Acts upstream of or within connective tissue replacement involved in inflammatory response wound healing. Located in basement membrane. Is expressed in several structures, including early conceptus; hindlimb cartilage condensation; jaw; reproductive system; and skeletal system. Orthologous to human TIMP1 (TIMP metallopeptidase inhibitor 1).

Rat Timp1 Target Information

  • Target Symbol: Timp1, TIMP metallopeptidase inhibitor 1
  • Alias:
    • metalloproteinase inhibitor 1
    • Timp
    • TIMP-1
    • tissue inhibitor of metallopeptidase 1
    • tissue inhibitor of metalloproteinase 1
    • tissue inhibitor of metalloproteinases 1
  • NCBI_Gene: 116510
  • UniProtKB: P30120

Rat Timp1 Predicted Functions

Enables protease binding activity. Involved in several processes, including cartilage development; response to peptide hormone; and wound healing. Located in basement membrane. Used to study degenerative disc disease. Biomarker of cholestasis; gastric ulcer; heart disease (multiple); lung disease (multiple); and vascular disease (multiple). Orthologous to human TIMP1 (TIMP metallopeptidase inhibitor 1).

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