EasyGo!™ Mouse IL-1β One-Step ELISA Kit

EK201BEGA

EasyGo!™ Mouse IL-1β One-Step ELISA Kit

$294.00 – $420.00

Related Target
Speciesmouse
Sample TypeSerum, plasma
Sample VolumeSerum, plasma: 5 μL
Sensitivity40.45 pg/mL
Array Range46.87 pg/mL - 3000 pg/mL
Assay Time1.5h
Recovery97% - 114%
Average Recovery105%

ELISA Kit Detail Information

Related Target
Speciesmouse
Sample TypeSerum, plasma
Sample VolumeSerum, plasma: 5 μL
Sensitivity40.45 pg/mL
Array Range46.87 pg/mL - 3000 pg/mL
Assay Time1.5h
Recovery97% - 114%
Average Recovery105%
Intra Precision6.4% - 7.9%
Inter Precision3.5% - 4.3%
PlateDetachable 96-well Plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ Blue Ice Transport
Components1× Washing Buffer
1× Assay Buffer
Stop Solution
Substrate TMB
Standard
Microplate Wells
Assay PrincipleThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-1B has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for IL-1B (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-1B present is bound by the immobilized antibody and detect antibody, of which the latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-1B bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader.

Related Targets

IL1B

IL1B Target Infomation Overview

  • Target Symbol: IL1B, interleukin 1 beta
  • Gene Groups: Interleukins
  • Alias: IL1F2; IL-1B; IL1-BETA

IL1B, interleukin 1 beta Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human IL1B Target Information

  • Target Symbol: IL1B, interleukin 1 beta
  • Alias:
    • catabolin
    • IL-1
    • IL-1 beta
    • IL1-BETA
    • IL1beta
    • IL1F2
    • interleukin 1, beta
    • interleukin 1beta
    • interleukin-1 beta
    • preinterleukin 1 beta
    • pro-interleukin-1-beta
  • NCBI_Gene: 3553
  • UniProtKB: P01584

Human IL1B Predicted Functions

Enables cytokine activity; integrin binding activity; and protein domain specific binding activity. Involved in several processes, including positive regulation of cytokine production; positive regulation of nitrogen compound metabolic process; and regulation of signal transduction. Acts upstream of or within cellular response to organic substance and cellular response to xenobiotic stimulus. Located in extracellular space. Implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); bronchial disease (multiple); lung disease (multiple); and nose disease (multiple).

Mouse Il1b Target Information

Mouse Il1b Predicted Functions

Enables cytokine activity. Involved in several processes, including negative regulation of cell communication; regulation of defense response; and regulation of lipid metabolic process. Acts upstream of or within several processes, including ectopic germ cell programmed cell death; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Located in extracellular space and secretory granule. Is expressed in several structures, including dorsal aorta; early conceptus; hemolymphoid system; integument; and reproductive system. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Orthologous to human IL1B (interleukin 1 beta).

Rat Il1b Target Information

Rat Il1b Predicted Functions

Enables cytokine activity. Involved in several processes, including positive regulation of intracellular signal transduction; regulation of gene expression; and regulation of neurogenesis. Located in extracellular space. Used to study several diseases, including artery disease (multiple); brain disease (multiple); glomerulonephritis (multiple); interstitial lung disease (multiple); and kidney failure (multiple). Biomarker of several diseases, including artery disease (multiple); auditory system disease (multiple); brain disease (multiple); kidney failure (multiple); and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Orthologous to human IL1B (interleukin 1 beta).

Products with the Same Target - IL1B

Rat IL-1β High Sensitivity ELISA Kit

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Rat IL-1β ELISA Kit

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Mouse IL-1β High Sensitivity ELISA Kit

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Mouse IL-1β ELISA Kit

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Human IL-1β High Sensitivity ELISA Kit

$266.00 – $380.00

Human IL-1β ELISA Kit

$245.00 – $350.00

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Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.

Continue Reading Competitive ELISA: Principles, Methods, and Key Differences

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