EK981EGA

EasyGo!™ Human/Mouse/Rat TGF-β1 One-Step ELISA Kit

$294.00$420.00

Related Target
Specieshuman,mouse,rat
Sample TypeSerum, plasma
Sample VolumeSerum, plasma: 40 μL
Sensitivity10.76 pg/mL
Array Range15.625 pg/mL - 1000 pg/mL
Assay Time1.5h
Recovery75% - 116%
Average Recovery94%

ELISA Kit Detail Information

Related Target
Specieshuman,mouse,rat
Sample TypeSerum, plasma
Sample VolumeSerum, plasma: 40 μL
Sensitivity10.76 pg/mL
Array Range15.625 pg/mL - 1000 pg/mL
Assay Time1.5h
Recovery75% - 116%
Average Recovery94%
Intra Precision5.8% - 9.1%
Inter Precision4.3% - 6.9%
PlateDetachable 96-well Plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ Blue Ice Transport
Components1× Washing Buffer
1× Assay Buffer
Stop Solution
Substrate TMB
Standard
Microplate Wells
HCl
NaOH
Assay PrincipleThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Human/Mouse/Rat TGF-β1 has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for TGF-β1 (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then TGF-β1 present is bound by the immobilized antibody and detect antibody, of which the latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of TGF-β1 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader.

Related Targets

TGFB1

TGFB1 Target Infomation Overview

  • Target Symbol: TGFB1, transforming growth factor beta 1
  • Gene Groups: Transforming growth factor beta family
  • Alias: CED; TGFbeta
  • Previous Names: TGFB; DPD1
  • Alias Names: Camurati-Engelmann disease; prepro-transforming growth factor beta-1; Diaphyseal dysplasia 1, progressive; transforming growth factor, beta 1

TGFB1, transforming growth factor beta 1 Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human TGFB1 Target Information

  • Target Symbol: TGFB1, transforming growth factor beta 1
  • Alias:
    • CED
    • DPD1
    • IBDIMDE
    • LAP
    • latency-associated peptide
    • prepro-transforming growth factor beta-1
    • TGF-beta 1 protein
    • TGF-beta-1
    • TGF-beta1
    • TGFB
    • TGFbeta
    • transforming growth factor beta-1
    • transforming growth factor beta-1 proprotein
    • transforming growth factor beta1
  • NCBI_Gene: 7040
  • UniProtKB: P01137

Human TGFB1 Predicted Functions

Enables several functions, including antigen binding activity; identical protein binding activity; and transforming growth factor beta receptor binding activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Acts upstream of or within epithelial to mesenchymal transition; negative regulation of gene expression; and positive regulation of isotype switching to IgA isotypes. Located in several cellular components, including cell surface; extracellular space; and nucleus. Colocalizes with microvillus. Implicated in several diseases, including Behcet’s disease; Camurati-Engelmann disease; aphthous stomatitis; artery disease (multiple); and lung disease (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); carcinoma (multiple); hematologic cancer (multiple); and lung disease (multiple).

Mouse Tgfb1 Target Information

Mouse Tgfb1 Predicted Functions

Enables protein serine/threonine kinase activator activity and transforming growth factor beta receptor binding activity. Involved in several processes, including circulatory system development; positive regulation of leukocyte differentiation; and regulation of cytokine production. Acts upstream of or within several processes, including animal organ development; positive regulation of histone modification; and regulation of protein localization to nucleus. Located in cytoplasm; extracellular matrix; and extracellular space. Is expressed in several structures, including alimentary system; embryo mesenchyme; genitourinary system; heart; and skin. Used to study Sjogren’s syndrome. Human ortholog(s) of this gene implicated in several diseases, including Behcet’s disease; Camurati-Engelmann disease; aphthous stomatitis; artery disease (multiple); and lung disease (multiple). Orthologous to human TGFB1 (transforming growth factor beta 1).

Rat Tgfb1 Target Information

  • Target Symbol: Tgfb1, transforming growth factor, beta 1
  • Alias:
    • TGF-beta-1
    • Tgfb
    • transforming growth factor beta-1
    • transforming growth factor beta-1 proprotein
    • transforming growth factor, beta-1
  • NCBI_Gene: 59086
  • UniProtKB: P17246

Rat Tgfb1 Predicted Functions

Enables identical protein binding activity and protein N-terminus binding activity. Involved in several processes, including animal organ development; cellular response to hormone stimulus; and regulation of epithelial cell proliferation. Acts upstream of or within positive regulation of smooth muscle cell differentiation. Located in several cellular components, including cell surface; neuronal cell body; and secretory granule. Used to study chronic obstructive pulmonary disease; familial hyperlipidemia; hypertension; proteinuria; and pulmonary fibrosis. Biomarker of several diseases, including allergic rhinitis; artery disease (multiple); cerebrovascular disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including Behcet’s disease; Camurati-Engelmann disease; aphthous stomatitis; artery disease (multiple); and lung disease (multiple). Orthologous to human TGFB1 (transforming growth factor beta 1).

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