EasyGo!™ Human/Mouse/Rat TGF-β1 One-Step ELISA Kit
$294.00 – $420.00
| Related Target | |
|---|---|
| Species | human,mouse,rat |
| Sample Type | Serum, plasma |
| Sample Volume | Serum, plasma: 40 μL |
| Sensitivity | 10.76 pg/mL |
| Array Range | 15.625 pg/mL - 1000 pg/mL |
| Assay Time | 1.5h |
| Recovery | 75% - 116% |
| Average Recovery | 94% |
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | human,mouse,rat |
| Sample Type | Serum, plasma |
| Sample Volume | Serum, plasma: 40 μL |
| Sensitivity | 10.76 pg/mL |
| Array Range | 15.625 pg/mL - 1000 pg/mL |
| Assay Time | 1.5h |
| Recovery | 75% - 116% |
| Average Recovery | 94% |
| Intra Precision | 5.8% - 9.1% |
| Inter Precision | 4.3% - 6.9% |
| Plate | Detachable 96-well Plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ Blue Ice Transport |
| Components | 1× Washing Buffer 1× Assay Buffer Stop Solution Substrate TMB Standard Microplate Wells HCl NaOH |
| Assay Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Human/Mouse/Rat TGF-β1 has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for TGF-β1 (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then TGF-β1 present is bound by the immobilized antibody and detect antibody, of which the latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of TGF-β1 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader. |
Related Targets
TGFB1
TGFB1 Target Infomation Overview
- Target Symbol: TGFB1, transforming growth factor beta 1
- Gene Groups: Transforming growth factor beta family
- Alias: CED; TGFbeta
- Previous Names: TGFB; DPD1
- Alias Names: Camurati-Engelmann disease; prepro-transforming growth factor beta-1; Diaphyseal dysplasia 1, progressive; transforming growth factor, beta 1
TGFB1, transforming growth factor beta 1 Target Infomation by Species
- Human
- Mouse
- Rat
Human TGFB1 Target Information
- Target Symbol: TGFB1, transforming growth factor beta 1
- Alias:
- CED
- DPD1
- IBDIMDE
- LAP
- latency-associated peptide
- prepro-transforming growth factor beta-1
- TGF-beta 1 protein
- TGF-beta-1
- TGF-beta1
- TGFB
- TGFbeta
- transforming growth factor beta-1
- transforming growth factor beta-1 proprotein
- transforming growth factor beta1
- NCBI_Gene: 7040
- UniProtKB: P01137
Human TGFB1 Predicted Functions
Enables several functions, including antigen binding activity; identical protein binding activity; and transforming growth factor beta receptor binding activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Acts upstream of or within epithelial to mesenchymal transition; negative regulation of gene expression; and positive regulation of isotype switching to IgA isotypes. Located in several cellular components, including cell surface; extracellular space; and nucleus. Colocalizes with microvillus. Implicated in several diseases, including Behcet’s disease; Camurati-Engelmann disease; aphthous stomatitis; artery disease (multiple); and lung disease (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); carcinoma (multiple); hematologic cancer (multiple); and lung disease (multiple).
Mouse Tgfb1 Target Information
- Target Symbol: Tgfb1, transforming growth factor, beta 1
- Alias:
- TGF-beta 1
- TGF-beta1
- Tgfb
- Tgfb-1
- TGFbeta1
- NCBI_Gene: 21803
Mouse Tgfb1 Predicted Functions
Enables protein serine/threonine kinase activator activity and transforming growth factor beta receptor binding activity. Involved in several processes, including circulatory system development; positive regulation of leukocyte differentiation; and regulation of cytokine production. Acts upstream of or within several processes, including animal organ development; positive regulation of histone modification; and regulation of protein localization to nucleus. Located in cytoplasm; extracellular matrix; and extracellular space. Is expressed in several structures, including alimentary system; embryo mesenchyme; genitourinary system; heart; and skin. Used to study Sjogren’s syndrome. Human ortholog(s) of this gene implicated in several diseases, including Behcet’s disease; Camurati-Engelmann disease; aphthous stomatitis; artery disease (multiple); and lung disease (multiple). Orthologous to human TGFB1 (transforming growth factor beta 1).
Rat Tgfb1 Target Information
- Target Symbol: Tgfb1, transforming growth factor, beta 1
- Alias:
- TGF-beta-1
- Tgfb
- transforming growth factor beta-1
- transforming growth factor beta-1 proprotein
- transforming growth factor, beta-1
- NCBI_Gene: 59086
- UniProtKB: P17246
Rat Tgfb1 Predicted Functions
Enables identical protein binding activity and protein N-terminus binding activity. Involved in several processes, including animal organ development; cellular response to hormone stimulus; and regulation of epithelial cell proliferation. Acts upstream of or within positive regulation of smooth muscle cell differentiation. Located in several cellular components, including cell surface; neuronal cell body; and secretory granule. Used to study chronic obstructive pulmonary disease; familial hyperlipidemia; hypertension; proteinuria; and pulmonary fibrosis. Biomarker of several diseases, including allergic rhinitis; artery disease (multiple); cerebrovascular disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including Behcet’s disease; Camurati-Engelmann disease; aphthous stomatitis; artery disease (multiple); and lung disease (multiple). Orthologous to human TGFB1 (transforming growth factor beta 1).
Products with the Same Target - TGFB1
Citations for EasyGo!™ Human/Mouse/Rat TGF-β1 One-Step ELISA Kit
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Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
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ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
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