Human Clusterin ELISA Kit

$350.00$450.00

SPECIES Human
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 100 μL (diluent)
Sensitivity 8.38 pg/mL
Range 0.25 ng/mL – 16 ng/mL
Assay Time 3.5 h
Recovery 76% – 117%
Average Recovery 0.98
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 100 μL (diluent)
Sensitivity 8.38 pg/mL
Range 0.25 ng/mL – 16 ng/mL
Assay Time 3.5 h
Recovery 76% – 117%
Average Recovery 0.98
Intra Precision 3.8 % – 6.2%
Inter-Precision 6.9 % – 9.0%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with mouse anti-human Clusterin capture antibody
Human Clusterin freeze-dried standard
Human Clusterin detect Antibody
Streptavidin-HRP
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Standard Diluent
Assay Principle This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Clusterin antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Clusterin present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Clusterin in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

6.2
Assessment of osteopontin as an early nephrotoxicity indicator in human renal proximal tubule cells and its application in evaluating lanthanum-induced nephrotoxicity
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

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