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Cortisol Competitive ELISA Kit
$350.00 – $450.00
| SPECIES | Human Mouse Rat |
|---|---|
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 100 μL |
| Sensitivity | 66.43 pg/mL |
| Range | 0.12 ng/mL – 15 ng/mL |
| Assay Time | 3.5 h |
| Recovery | 80% – 130% |
| Average Recovery | 1.18 |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 100 μL |
| Sensitivity | 66.43 pg/mL |
| Range | 0.12 ng/mL – 15 ng/mL |
| Assay Time | 3.5 h |
| Recovery | 80% – 130% |
| Average Recovery | 1.18 |
| Intra Precision | 4.3% – 4.6% |
| Inter-Precision | 6.4% – 7.8% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Competitive monoclonal antibody Cortisol competitive freeze-dried standard Competitive detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes a competitive enzyme-linked immunosorbent assay (ELISA) technique. Rabbit anti-mouse antibody is precoated on a high-affinity ELISA plate. Cortisol-specific monoclonal antibodies are added to the wells of the plate and incubated, allowing them to bind to the solid-phase antibodies. After washing, HRP-labeled cortisol and unlabeled cortisol or samples are added, competing for limited binding sites on the monoclonal antibody. After washing to remove unbound substances, a chromogenic substrate is added, and the intensity of the color reaction is inversely proportional to the concentration of cortisol in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). This assay kit has been validated for human, mouse, and rat serum samples, but cortisol from other species is also expected to be detectable. |
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