Mouse BMP-9 ELISA Kit

$350.00$450.00

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TARGETSGDF2
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL(diluent)
Sensitivity0.77 pg/mL
Range23.4 pg/mL – 1500 pg/mL
Assay Time3.5 h
Recovery72% – 123%
Average Recovery1.02
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL(diluent)
Sensitivity0.77 pg/mL
Range23.4 pg/mL – 1500 pg/mL
Assay Time3.5 h
Recovery72% – 123%
Average Recovery1.02
Intra Precision2.6% – 3.8%
Inter-Precision4.4% – 5.0%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti- BMP-9 monoclonal antibody
Mouse BMP-9 freeze-dried standard
BMP-9 detect Antibody
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse BMP-9 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, BMP-9 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of BMP-9 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

GDF2

GDF2 Target Infomation Overview

  • Target Symbol: GDF2, growth differentiation factor 2
  • Gene Groups: Transforming growth factor beta superfamily
  • Alias: BMP-9; BMP9

GDF2, growth differentiation factor 2 Target Infomation by Species

HumanMouseRat

Human GDF2 Target Information

  • Target Symbol: GDF2, growth differentiation factor 2
  • Alias:
    • BMP-9
    • BMP9
    • bone morphogenetic protein 9
    • GDF-2
    • growth/differentiation factor 2
    • HHT5
  • NCBI_Gene: 2658
  • UniProtKB: Q9UK05

Human GDF2 Predicted Functions

Predicted to enable cytokine activity. Involved in several processes, including positive regulation of macromolecule metabolic process; regulation of endothelial cell proliferation; and transmembrane receptor protein serine/threonine kinase signaling pathway. Located in extracellular exosome. Implicated in hereditary hemorrhagic telangiectasia.

Mouse Gdf2 Target Information

Mouse Gdf2 Predicted Functions

Predicted to enable cytokine activity. Involved in several processes, including blood vessel morphogenesis; positive regulation of BMP signaling pathway; and positive regulation of endothelial cell differentiation. Acts upstream of or within osteoblast differentiation; positive regulation of gene expression; and positive regulation of transcription by RNA polymerase II. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Is expressed in several structures, including central nervous system; ileum; interphalangeal joint; liver; and male reproductive gland or organ. Human ortholog(s) of this gene implicated in hereditary hemorrhagic telangiectasia. Orthologous to human GDF2 (growth differentiation factor 2).

Rat Gdf2 Target Information

  • Target Symbol: Gdf2, growth differentiation factor 2
  • Alias:
    • growth/differentiation factor 2
    • LOC290921
    • similar to Growth/differentiation factor 2 precursor (GDF-2) (Bone morphogenetic protein 9) (BMP-9)
  • NCBI_Gene: 290921
  • UniProtKB: M0RB87

Rat Gdf2 Predicted Functions

Predicted to enable cytokine activity. Predicted to be involved in several processes, including positive regulation of transmembrane receptor protein serine/threonine kinase signaling pathway; regulation of endothelial cell proliferation; and transmembrane receptor protein serine/threonine kinase signaling pathway. Predicted to act upstream of or within osteoblast differentiation; positive regulation of gene expression; and positive regulation of transcription by RNA polymerase II. Predicted to be active in extracellular space. Human ortholog(s) of this gene implicated in hereditary hemorrhagic telangiectasia. Orthologous to human GDF2 (growth differentiation factor 2).

GDF2 Target News

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