Human Furin ELISA Kit

$350.00$450.00

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SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL (diluent)
Sensitivity53.63 pg/mL
Range109.4 pg/mL – 7000 pg/mL
Assay Time3.5 h
Recovery81% – 120%
Average Recovery0.97
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL (diluent)
Sensitivity53.63 pg/mL
Range109.4 pg/mL – 7000 pg/mL
Assay Time3.5 h
Recovery81% – 120%
Average Recovery0.97
Intra Precision7.3% – 7.9%
Inter-Precision4.5% – 8.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with mouse anti-human Furin capture antibody
Human Furin freeze-dried standard
Human Furin detect Antibody
Streptavidin-HRP
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Standard Diluent
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Furin antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Furin present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of Furin in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

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