Mouse Fas/TNFRSF6/CD95 ELISA Kit

$350.00$450.00

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TARGETSFAS
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity2.34 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery75% – 96%
Average Recovery0.85
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity2.34 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery75% – 96%
Average Recovery0.85
Intra Precision2.1% – 7.6%
Inter-Precision2.1%-7.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Fas monoclonal antibody
Mouse Fas freeze-dried standard
Fas detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse Fas antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Fas present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Fas in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

FAS

FAS Target Infomation Overview

  • Target Symbol: FAS, Fas cell surface death receptor
  • Gene Groups: CD molecules; Tumor necrosis factor receptor superfamily; Death inducing signaling complex
  • Alias: CD95; APO-1
  • Previous Names: FAS1; APT1; TNFRSF6
  • Alias Names: TNF receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6; Fas (TNF receptor superfamily, member 6)

FAS, Fas cell surface death receptor Target Infomation by Species

HumanMouseRat

Human FAS Target Information

  • Target Symbol: FAS, Fas cell surface death receptor
  • Alias:
    • ALPS1A
    • APO-1
    • apo-1 antigen
    • APO-1 cell surface antigen
    • apoptosis antigen 1
    • apoptosis signaling receptor FAS
    • apoptosis-mediating surface antigen FAS
    • APT1
    • CD95
    • CD95 antigen
    • Delta Fas/APO-1/CD95
    • Fas (TNF receptor superfamily, member 6)
    • FAS 827dupA
    • Fas AMA
    • Fas antigen
    • FAS receptor variant 9
    • FAS1
    • FASLG receptor
    • FASTM
    • mutant tumor necrosis receptor superfamily member 6
    • RP11-399O19.9
    • TNF receptor superfamily member 6
    • TNFRSF6
    • tumor necrosis factor receptor superfamily member 6
    • tumor necrosis factor receptor superfamily, member 6
  • NCBI_Gene: 355
  • UniProtKB: P25445

Human FAS Predicted Functions

Enables calmodulin binding activity; identical protein binding activity; and kinase binding activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of cellular protein metabolic process; and regulation of signal transduction. Acts upstream of or within apoptotic process. Located in several cellular components, including cell surface; membrane raft; and nuclear body. Part of CD95 death-inducing signaling complex. Implicated in several diseases, including autoimmune disease (multiple); cystic fibrosis; hematologic cancer (multiple); pre-eclampsia (multiple); and urinary system cancer (multiple). Biomarker of several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); and neurodegenerative disease (multiple).

Mouse Fas Target Information

Mouse Fas Predicted Functions

Enables identical protein binding activity. Involved in positive regulation of protein-containing complex assembly. Acts upstream of or within several processes, including extrinsic apoptotic signaling pathway; hematopoietic or lymphoid organ development; and regulation of hemopoiesis. Located in external side of plasma membrane and extracellular region. Part of CD95 death-inducing signaling complex. Is expressed in several structures, including alimentary system; brain; genitourinary system; hemolymphoid system gland; and liver and biliary system. Used to study Sjogren’s syndrome; autoimmune lymphoproliferative syndrome; systemic lupus erythematosus; and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); cystic fibrosis; hematologic cancer (multiple); pre-eclampsia (multiple); and urinary system cancer (multiple). Orthologous to human FAS (Fas cell surface death receptor).

Rat Fas Target Information

  • Target Symbol: Fas, Fas cell surface death receptor
  • Alias:
    • apo-1 antigen
    • apoptosis-mediating surface antigen FAS
    • CD95 antigen
    • Fas (TNF receptor superfamily, member 6)
    • Fas antigen (ATP1)
    • Fas receptor
    • FASLG receptor
    • Tnfrsf6
    • tumor necrosis factor receptor superfamily member 6
    • Tumor necrosis factor receptor superfamily, member 6
  • NCBI_Gene: 246097

Rat Fas Predicted Functions

Enables protease binding activity and tumor necrosis factor-activated receptor activity. Involved in several processes, including cellular response to cytokine stimulus; cellular response to estrogen stimulus; and nervous system development. Located in several cellular components, including apical dendrite; apical plasma membrane; and sarcolemma. Part of CD95 death-inducing signaling complex. Used to study IgA glomerulonephritis; brain glioma; pre-malignant neoplasm; retinal detachment; and transient cerebral ischemia. Biomarker of several diseases, including abdominal obesity-metabolic syndrome 1; artery disease (multiple); brain ischemia (multiple); eye disease (multiple); and liver disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); cystic fibrosis; hematologic cancer (multiple); pre-eclampsia (multiple); and urinary system cancer (multiple). Orthologous to human FAS (Fas cell surface death receptor).

FAS Target News

    Multiple Sclerosis
    Systemic Lupus Erythematosus

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