Product Category
Newest Products
Mouse sIL-2Rα/CD25 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 100 μL(diluent) |
| Sensitivity | 0.31 pg/mL |
| Range | 7.81 pg/mL – 500 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 73% – 102% |
| Average Recovery | 0.82 |
| Intra Precision | 2.6% – 3.5% |
| Inter-Precision | 2.5%-4.2% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-sIL-2Rα monoclonal antibody Mouse sIL-2Rα freeze-dried standard Mouse sIL-2Rα detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse sIL-2Rα antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, sIL-2Rα present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of sIL-2Rα in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
IL2RA
IL2RA Target Infomation Overview
- Target Symbol: IL2RA, interleukin 2 receptor subunit alpha
- Gene Groups: CD molecules; Interleukin receptors; Sushi domain containing
- Alias: CD25
- Previous Names: IL2R; IDDM10
- Alias Names: insulin-dependent diabetes mellitus 10; interleukin 2 receptor, alpha
IL2RA, interleukin 2 receptor subunit alpha Target Infomation by Species
HumanMouseRat
Human IL2RA Target Information
- Target Symbol: IL2RA, interleukin 2 receptor subunit alpha
- Alias:
- CD25
- IDDM10
- IL-2 receptor subunit alpha
- IL-2-RA
- IL-2R subunit alpha
- IL2-RA
- IL2R
- IMD41
- insulin-dependent diabetes mellitus 10
- interleukin 2 receptor, alpha
- interleukin 2 receptor, alpha chain
- interleukin-2 receptor subunit alpha
- p55
- TAC antigen
- TCGFR
- NCBI_Gene: 3559
- UniProtKB: P01589
Human IL2RA Predicted Functions
Contributes to interleukin-2 receptor activity. Involved in regulation of T cell tolerance induction. Predicted to be located in plasma membrane. Predicted to be part of interleukin-2 receptor complex. Implicated in immunodeficiency 41; lymphopenia; multiple sclerosis; type 1 diabetes mellitus; and type 1 diabetes mellitus 10. Biomarker of COVID-19; Hantavirus hemorrhagic fever with renal syndrome; and idiopathic pulmonary fibrosis.
Mouse Il2ra Target Information
- Target Symbol: Il2ra, interleukin 2 receptor, alpha chain
- Alias:
- CD25
- IL-2R alpha chain
- Il2r
- interleukin 2 receptor
- Ly-43
- lymphocyte antigen 43
- NCBI_Gene: 16184
Mouse Il2ra Predicted Functions
Enables interleukin-2 binding activity and interleukin-2 receptor activity. Acts upstream of or within several processes, including Notch signaling pathway; activation-induced cell death of T cells; and regulation of T cell proliferation. Located in external side of plasma membrane. Is expressed in bone marrow. Used to study Sjogren’s syndrome and inflammatory bowel disease. Human ortholog(s) of this gene implicated in immunodeficiency 41; lymphopenia; multiple sclerosis; type 1 diabetes mellitus; and type 1 diabetes mellitus 10. Orthologous to human IL2RA (interleukin 2 receptor subunit alpha).
Rat Il2ra Target Information
- Target Symbol: Il2ra, interleukin 2 receptor subunit alpha
- Alias:
- IL-2 receptor alpha subunit
- IL-2 receptor subunit alpha
- IL-2-RA
- IL-2R subunit alpha
- IL2-RA
- IL2RAC
- interleukin 2 receptor, alpha
- interleukin 2 receptor, alpha chain
- interleukin-2 receptor alpha chain
- interleukin-2 receptor subunit alpha
- NCBI_Gene: 25704
- UniProtKB: P26897
Rat Il2ra Predicted Functions
Enables interleukin-2 receptor activity. Involved in inflammatory response to antigenic stimulus; positive regulation of T cell differentiation; and positive regulation of T cell proliferation. Located in cell surface. Used to study Korean hemorrhagic fever; asthma; and diabetes mellitus. Human ortholog(s) of this gene implicated in immunodeficiency 41; lymphopenia; multiple sclerosis; type 1 diabetes mellitus; and type 1 diabetes mellitus 10. Orthologous to human IL2RA (interleukin 2 receptor subunit alpha).
