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Human Growth Hormone/GH ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 20 μL |
| Sensitivity | 0.62 pg/mL |
| Range | 62.5 pg/mL – 4000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 80% – 124% |
| Average Recovery | 1.05 |
| Intra Precision | 4.3% – 5.9% |
| Inter-Precision | 5.5% – 6.9% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Growth Hormone/GH monoclonal antibody Human Growth Hormone/GH freeze-dried standard Growth Hormone/GH detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Growth Hormone antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the Growth Hormone present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Growth Hormone in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
GH1
GH1 Target Infomation Overview
- Target Symbol: GH1, growth hormone 1
- Gene Groups: Growth hormone family
- Alias: GH-N; GHN; GH; hGH-N
- Alias Names: pituitary growth hormone; somatotropin
GH1, growth hormone 1 Target Infomation by Species
HumanMouseRat
Human GH1 Target Information
- Target Symbol: GH1, growth hormone 1
- Alias:
- GH
- GH-N
- GHB5
- GHN
- growth hormone B5
- hGH-N
- IGHD1A
- IGHD1B
- IGHD2
- pituitary growth hormone
- somatotropin
- NCBI_Gene: 2688
- UniProtKB: P01241
Human GH1 Predicted Functions
Enables growth factor activity; growth hormone receptor binding activity; and prolactin receptor binding activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of peptidyl-tyrosine phosphorylation; and positive regulation of signal transduction. Acts upstream of or within positive regulation of glucose transmembrane transport. Located in extracellular space. Part of growth hormone receptor complex. Colocalizes with collagen-containing extracellular matrix. Implicated in several diseases, including anemia (multiple); autoimmune disease of endocrine system (multiple); diabetic retinopathy; isolated growth hormone deficiency (multiple); and progeria. Biomarker of diabetes insipidus; diabetes mellitus (multiple); osteoarthritis; and severe acute respiratory syndrome.
Mouse Gh Target Information
- Target Symbol: Gh, growth hormone
- Alias:
- 无
- NCBI_Gene: 14599
Mouse Gh Predicted Functions
Enables growth hormone receptor binding activity. Acts upstream of or within cellular response to insulin stimulus; regulation of growth; and response to food. Located in several cellular components, including extracellular space; secretory granule; and trans-Golgi network. Is expressed in brain; early conceptus; gut; hemolymphoid system; and male reproductive system. Used to study isolated growth hormone deficiency type II. Human ortholog(s) of this gene implicated in several diseases, including anemia (multiple); autoimmune disease of endocrine system (multiple); diabetic retinopathy; isolated growth hormone deficiency (multiple); and progeria. Orthologous to several human genes including GH1 (growth hormone 1).
Rat Gh1 Target Information
- Target Symbol: Gh1, growth hormone 1
- Alias:
- Gh
- Ghb1
- growth hormone b1
- RNGHGP
- somatotropin
- NCBI_Gene: 24391
Rat Gh1 Predicted Functions
Enables hormone activity. Involved in several processes, including cellular response to alkaline pH; cellular response to thyroid hormone stimulus; and generation of neurons. Located in several cellular components, including collagen-containing extracellular matrix; mitochondrion; and secretory granule. Used to study isolated growth hormone deficiency and type 1 diabetes mellitus. Biomarker of congenital hypothyroidism; osteoporosis; and type 2 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including anemia (multiple); autoimmune disease of endocrine system (multiple); diabetic retinopathy; isolated growth hormone deficiency (multiple); and progeria. Orthologous to several human genes including GH1 (growth hormone 1).
