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Human IL-16 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | Serum, plasma: 10 μL；cell culture supernatant: 100 μL |
| Sensitivity | 5.31 pg/mL |
| Range | 15.63 pg/mL – 1000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 80% – 109% |
| Average Recovery | 0.96 |
| Intra Precision | 2.8%-6.6% |
| Inter-Precision | 2.2% – 5.7% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-16 monoclonal antibody Human IL-16 freeze-dried standard IL-16 detect Antibody Standard Diluent HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-16 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-16 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-16 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
IL16
IL16 Target Infomation Overview
- Target Symbol: IL16, interleukin 16
- Gene Groups: Interleukins; PDZ domain containing
- Alias: LCF; IL-16; prIL-16; HsT19289; FLJ42735; FLJ16806
- Alias Names: prointerleukin 16; lymphocyte chemoattractant factor; interleukin 16 (lymphocyte chemoattractant factor)
IL16, interleukin 16 Target Infomation by Species
HumanMouseRat
Human IL16 Target Information
- Target Symbol: IL16, interleukin 16
- Alias:
- FLJ16806
- FLJ42735
- FLJ44234
- HsT19289
- IL-16
- LCF
- lymphocyte chemoattractant factor
- neuronal interleukin 16
- NIL16
- prIL-16
- PRIL16
- pro-interleukin-16
- prointerleukin 16
- NCBI_Gene: 3603
- UniProtKB: Q14005
Human IL16 Predicted Functions
Enables CD4 receptor binding activity. Involved in positive regulation of cytokine production; positive regulation of inflammatory response; and regulation of calcium ion transport. Located in several cellular components, including Flemming body; focal adhesion; and nuclear speck. Implicated in asthma and prostate cancer. Biomarker of asthma; chronic fatigue syndrome; chronic obstructive pulmonary disease; malignant astrocytoma; and rhinitis.
Mouse Il16 Target Information
- Target Symbol: Il16, interleukin 16
- Alias:
- 无
- NCBI_Gene: 16170
Mouse Il16 Predicted Functions
Enables cytokine activity. Involved in cytokine-mediated signaling pathway. Acts upstream of or within several processes, including induction of positive chemotaxis; positive regulation of chemokine production; and positive regulation of neutrophil activation. Located in collagen-containing extracellular matrix. Is expressed in several structures, including foregut; jaw; limb; respiratory system; and vibrissa. Human ortholog(s) of this gene implicated in asthma and prostate cancer. Orthologous to human IL16 (interleukin 16).
Rat Il16 Target Information
- Target Symbol: Il16, interleukin 16
- Alias:
- Il16_mapped
- interleukin 16 (mapped)
- pro-interleukin-16
- NCBI_Gene: 116996
- UniProtKB: D4A4I9
Rat Il16 Predicted Functions
Predicted to enable CD4 receptor binding activity and cytokine activity. Predicted to be involved in several processes, including induction of positive chemotaxis; leukocyte chemotaxis; and positive regulation of cytokine production. Predicted to act upstream of or within positive regulation of chemokine production and positive regulation of neutrophil activation. Predicted to be located in several cellular components, including Flemming body; focal adhesion; and nuclear speck. Biomarker of borna disease; glioblastoma; and periodontal disease. Human ortholog(s) of this gene implicated in asthma and prostate cancer. Orthologous to human IL16 (interleukin 16).
