Human IL-16 ELISA Kit

$350.00$450.00

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TARGETSIL16
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 10 μL;cell culture supernatant: 100 μL
Sensitivity5.31 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery80% – 109%
Average Recovery0.96
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 10 μL;cell culture supernatant: 100 μL
Sensitivity5.31 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery80% – 109%
Average Recovery0.96
Intra Precision2.8%-6.6%
Inter-Precision2.2% – 5.7%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-16 monoclonal antibody
Human IL-16 freeze-dried standard
IL-16 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-16 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-16 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-16 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL16

IL16 Target Infomation Overview

  • Target Symbol: IL16, interleukin 16
  • Gene Groups: Interleukins; PDZ domain containing
  • Alias: LCF; IL-16; prIL-16; HsT19289; FLJ42735; FLJ16806
  • Alias Names: prointerleukin 16; lymphocyte chemoattractant factor; interleukin 16 (lymphocyte chemoattractant factor)

IL16, interleukin 16 Target Infomation by Species

HumanMouseRat

Human IL16 Target Information

  • Target Symbol: IL16, interleukin 16
  • Alias:
    • FLJ16806
    • FLJ42735
    • FLJ44234
    • HsT19289
    • IL-16
    • LCF
    • lymphocyte chemoattractant factor
    • neuronal interleukin 16
    • NIL16
    • prIL-16
    • PRIL16
    • pro-interleukin-16
    • prointerleukin 16
  • NCBI_Gene: 3603
  • UniProtKB: Q14005

Human IL16 Predicted Functions

Enables CD4 receptor binding activity. Involved in positive regulation of cytokine production; positive regulation of inflammatory response; and regulation of calcium ion transport. Located in several cellular components, including Flemming body; focal adhesion; and nuclear speck. Implicated in asthma and prostate cancer. Biomarker of asthma; chronic fatigue syndrome; chronic obstructive pulmonary disease; malignant astrocytoma; and rhinitis.

Mouse Il16 Target Information

Mouse Il16 Predicted Functions

Enables cytokine activity. Involved in cytokine-mediated signaling pathway. Acts upstream of or within several processes, including induction of positive chemotaxis; positive regulation of chemokine production; and positive regulation of neutrophil activation. Located in collagen-containing extracellular matrix. Is expressed in several structures, including foregut; jaw; limb; respiratory system; and vibrissa. Human ortholog(s) of this gene implicated in asthma and prostate cancer. Orthologous to human IL16 (interleukin 16).

Rat Il16 Target Information

Rat Il16 Predicted Functions

Predicted to enable CD4 receptor binding activity and cytokine activity. Predicted to be involved in several processes, including induction of positive chemotaxis; leukocyte chemotaxis; and positive regulation of cytokine production. Predicted to act upstream of or within positive regulation of chemokine production and positive regulation of neutrophil activation. Predicted to be located in several cellular components, including Flemming body; focal adhesion; and nuclear speck. Biomarker of borna disease; glioblastoma; and periodontal disease. Human ortholog(s) of this gene implicated in asthma and prostate cancer. Orthologous to human IL16 (interleukin 16).

IL16 Target News

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